Protein Info for HMPREF1181_RS10570 in Bacteroides stercoris CC31F

Annotation: sirohydrochlorin cobaltochelatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06180: CbiK
  • amino acids 40 to 292 (253 residues), 260 bits, E=2.3e-81
• PF01903: CbiX
  • amino acids 214 to 287 (74 residues), 27.9 bits, E=2.5e-10

Best Hits

KEGG orthology group: K02190, sirohydrochlorin cobaltochelatase [EC: 4.99.1.3] (inferred from 78% identity to bhl:Bache_2221)

Predicted SEED Role

"Sirohydrochlorin cobaltochelatase CbiK (EC 4.99.1.3)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 4.99.1.3)

MetaCyc Pathways

• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (12/15 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (24/36 steps found)
• siroheme biosynthesis (1/4 steps found)
• factor 430 biosynthesis (1/7 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.99.1.3

Use Curated BLAST to search for 4.99.1.3

Protein Sequence (316 amino acids)

>HMPREF1181_RS10570 sirohydrochlorin cobaltochelatase (Bacteroides stercoris CC31F)
MKTSLLSLLLAISLFCSAHEGGNFVSSDMLASMKPGEKAALLMVHFGTTHDDTRTQTIDA
INAQARKAFPDLEFREAYTSRIVIRRLKTRGVVKNTPLDALLQLRGEGYTHIIVQSTNII
DGVEMESLRRDVESVLPFFKEIRVSTPLLYSVEDAKKVTDILGQRLNASVQQSAKKKGKE
HFVLVGHGTYTPGTATYSQMDYMLKVAGFGNFHVGTIEGYPTFETMLAQLKAAKAKSVTL
VPFMFVAGDHAKNDIAGEWREMLEKEGYTVHVRMEGLGQIPEIQKIFVDHIRFGLKHRTQ
GIMEKKAVYAAGEKNE