Protein Info for HMPREF1181_RS09205 in Bacteroides stercoris CC31F

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 PF13328: HD_4" amino acids 44 to 194 (151 residues), 149.7 bits, see alignment E=1.8e-47 TIGR00691: RelA/SpoT family protein" amino acids 44 to 749 (706 residues), 651.3 bits, see alignment E=9.2e-200 PF01966: HD" amino acids 64 to 163 (100 residues), 32.5 bits, see alignment E=2.9e-11 PF04607: RelA_SpoT" amino acids 255 to 369 (115 residues), 124.7 bits, see alignment E=6.3e-40 PF02824: TGS" amino acids 412 to 471 (60 residues), 74.2 bits, see alignment 2.1e-24 PF19296: RelA_AH_RIS" amino acids 483 to 649 (167 residues), 32.7 bits, see alignment E=2.5e-11 PF13291: ACT_4" amino acids 674 to 749 (76 residues), 69.5 bits, see alignment E=9.5e-23

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 93% identity to bhl:Bache_1247)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (752 amino acids)

>HMPREF1181_RS09205 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Bacteroides stercoris CC31F)
MEESVSQKEKERAEEAMIEQAFQELLNDYLATKHRKRVEIITKAFNFANQAHKGIKRRSG
EPYIMHPIAVAKIVCNEIGLGSTSICAALLHDVVEDTDYTVEDIENIFGPKIAQIVDGLT
KISGGIFGDRASAQAENFKKLLLTMSDDIRVILIKIADRLHNMRTLGSMLPNKQFKIAGE
TLYIYAPLANRLGLYKIKTELENLSFRYEHPEEYREIEKKLEATAVERDKVFKEFTAPIR
AQLDKMGLKYRILARIKSIYSIWNKMQTKHVPFEEIYDLLAVRIIFEPRNMEEELNDCFN
IYVSISKIYKPHPDRLRDWVSHPKANGYQALHVTLMGNNGQWIEVQIRSERMNDVAEQGF
AAHWKYKEGGGSEDEGELDKWLRTIKEILDDPQPDAIDFLDTIKLNLFASEIFVFTPKGE
IKTMPQNSTALDFAFSLHTDIGSHCIGAKVNHKLVPLSHKLQSGDQVEVLTSKSQRVQPE
WEAYATTARARAKIASILRKEAKAHQKEGEAILADFLKNEDIRMDDSAIDKLTRLHNFHT
RDELFVAVGSKKLVLGDADRNAFKEKQSTNWKKFLTFSFGNKDNKDTKEQSEEKVPQEKI
NTKQILKLTEETIQKNYIMADCCHPIPGDDVLGYIDEQNRIIIHKRQCPVAARLKSSYGN
RIIATEWDTHKELSFLVTIYLKGIDSMGLLNEVTQVISRQLNVNIRKLTIETTDGIFEGK
IQLYVHDVDDVRSICDNLKQIQNIKQVTRIEG