Protein Info for HMPREF1181_RS08970 in Bacteroides stercoris CC31F

Annotation: bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 29 to 476 (448 residues), 290.6 bits, E=2e-90
• PF08245: Mur_ligase_M
  • amino acids 132 to 317 (186 residues), 90.3 bits, E=2.5e-29
• TIGR00492: alanine racemase
  • amino acids 483 to 840 (358 residues), 291.3 bits, E=9.5e-91
• PF01168: Ala_racemase_N
  • amino acids 487 to 708 (222 residues), 196.3 bits, E=9.1e-62
• PF00842: Ala_racemase_C
  • amino acids 717 to 840 (124 residues), 143 bits, E=6.1e-46

Best Hits

KEGG orthology group: K01775, alanine racemase [EC: 5.1.1.1] (inferred from 90% identity to bhl:Bache_1322)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10) / Alanine racemase (EC 5.1.1.1)" in subsystem Alanine biosynthesis or Pyruvate Alanine Serine Interconversions or Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 5.1.1.1, EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• alanine racemization (1/1 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• L-alanine degradation I (1/2 steps found)
• anaerobic energy metabolism (invertebrates, cytosol) (4/7 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• ansatrienin biosynthesis (1/9 steps found)
• cyclosporin A biosynthesis (1/15 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• D-Alanine metabolism
• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.10

Use Curated BLAST to search for 5.1.1.1 or 6.3.2.10

Protein Sequence (843 amino acids)

>HMPREF1181_RS08970 bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase (Bacteroides stercoris CC31F)
MSYTIEDIARIIGARRIGDRPAAIDWLLTDSRSLSFPEETLFFALPTKRNDGTRYIRDLY
ARGVRNFVVSEERLNEVGIGEPKVEDAARQETADTAAMQPAFNLLVVPNPLKALQKLAEQ
HRDRFQIPVIGITGSNGKTIVKEWLHQLLSPERMITRSPRSYNSQIGVPLSVWQMSEQTE
LAILEAGISEPGEMRALQNIIKPTIGILTNIGGAHQENFFSLQEKCMEKLALFKNCDVVI
YNGDDEFINNCVGKSMLAAREIAWSRKDMERPLYISKVEKQEDSTVISYRYLDMDNTFTL
PFIDDASIENSLNCLAACLYLMLPAEQITERMAKLEPVAMRLEVKEGKNGCLLINDSYNS
DLGSLDIALDFLYRRSQSKGLKRTLILSDILETGQNTPTLYRQVAQLVNSRGIERIIGVG
NEISSCAARFSIEKAFYPDTATLIEAIGRGELRLENEIILIKGARKFGFDALTEALEKKV
HETILEVNLGAMIANLNHYRGKLKPETKMVCMVKASAYGAGSYEIARTLQEHHVDYLAVA
VADEGSDLRKAGITASIIIMNPEMTAFKTMFDYKLEPEVYSFHLLDALIKEAEKEGITNF
PIHVKLDTGMHRLGFAPEDMPRLIERLKGQNAVIPRSVFSHFVGSDAQQFDAFTRKQIET
FEKASMLLQEAFPYKILRHICNSAGIERFPGAQFDMVRLGIGLYGISPIDNSIMHNVSTL
KTTILQIRDVPEEDTVGYSRKGHLTRPSRIAAIPIGYADGLNRHLGNGHAYCLVNGQRAP
YVGNICMDVCMIDVTDIDCKEGDSVEIFGDHLPITVLSDVLGTIPYEVLTSVSTRVKRVY
YQD