Protein Info for HMPREF1181_RS08495 in Bacteroides stercoris CC31F
Annotation: methylmalonyl-CoA mutase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to MUTB_PORGI: Methylmalonyl-CoA mutase large subunit (mutB) from Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 98% identity to bhl:Bache_3333)MetaCyc: 74% identical to methylmalonyl-CoA mutase large subunit (Chloroflexus aurantiacus)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]
Predicted SEED Role
"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)
MetaCyc Pathways
- 2-oxobutanoate degradation I (4/4 steps found)
- propanoyl CoA degradation I (3/3 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
- pyruvate fermentation to propanoate I (5/7 steps found)
- conversion of succinate to propanoate (2/3 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
- L-glutamate degradation VIII (to propanoate) (5/11 steps found)
- methylaspartate cycle (10/19 steps found)
- 3-hydroxypropanoate cycle (5/13 steps found)
- superpathway of L-methionine salvage and degradation (7/16 steps found)
- 3-hydroxypropanoate/4-hydroxybutanate cycle (5/18 steps found)
- superpathway of the 3-hydroxypropanoate cycle (5/18 steps found)
- crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.4.99.2
Use Curated BLAST to search for 5.4.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (715 amino acids)
>HMPREF1181_RS08495 methylmalonyl-CoA mutase (Bacteroides stercoris CC31F) MRKDFKNLDIYAAFQPANGAEWQKANGIKADWKTPEHIEVKPVYTKEDLEGMEHLDYAAG IPPYLRGPYSVMYTLRPWTIRQYAGFSTAEESNAFYRRNLASGQKGLSVAFDLATHRGYD PDHERVVGDVGKAGVSICSLENMKVLFDGIPLNKMSVSMTMNGAVLPIMAFYINAGLEQG AKLEEMAGTIQNDILKEFMVRNTYIYPPAFSMKIISDIFEYTSQKMPKFNSISISGYHMQ EAGATADIELAYTLADGLEYLRAGVAAGIDIDAFAPRLSFFWAIGTNHFMEIAKMRAARM LWAKIVKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTN ALDEAIALPTDFSARIARNTQIYIQEETYICKNVDPWGGSYYVESLTNELAHKAWELIQE IEKLGGMAKAIETGIPKMRIEEAAARTQARIDSGSQTIVGVNKYRLEKEAPIDILEIDNT AVRQEQIQNLKILKEGRDEAAVQKALDAITKCVETKEGNLLELAVEAARVRATLGEISYA CEKVVGRYKAVIRTISGVYSSESKNDSDFHRACELAEKFAKKEGRQPRIMVAKMGQDGHD RGAKVVATGYADCGFDVDMGPLFQTPAEAAREAVENDVHVVGVSSLAAGHKTLVPQIIEE LKKLGREDIVVIAGGVIPAQDYDFLYKAGVAAIFGPGTPVAKAACQILEILLDEE