Protein Info for HMPREF1181_RS07835 in Bacteroides stercoris CC31F

Annotation: NADP-specific glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 53 to 181 (129 residues), 171.5 bits, E=6.5e-55
• PF00208: ELFV_dehydrog
  • amino acids 198 to 443 (246 residues), 301.4 bits, E=5.5e-94

Best Hits

Swiss-Prot: 93% identical to DHE3_BACTN: Glutamate dehydrogenase (gdhA) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 95% identity to bfs:BF3409)

MetaCyc: 60% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2) / NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Arginine and Ornithine Degradation or Proline Synthesis (EC 1.4.1.2, EC 1.4.1.4)

MetaCyc Pathways

• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-alanine degradation II (to D-lactate) (2/3 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• methylaspartate cycle (10/19 steps found)
• 4-aminobutanoate degradation V (1/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (2/10 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.2, 1.4.1.4

Use Curated BLAST to search for 1.4.1.2 or 1.4.1.4

Protein Sequence (445 amino acids)

>HMPREF1181_RS07835 NADP-specific glutamate dehydrogenase (Bacteroides stercoris CC31F)
MNAAKVLEDLKRRFPNEPEYHQAVEEVLSTIEEEYNKHPEFDKANLIERLCIPDRVYQFR
VTWVDDKGNVQTNMGYRVQHNNAIGPYKGGIRFHASVNLSILKFLAFEQTFKNSLTTLPM
GGGKGGSDFSPRGKSNAEVMRFVQAFMLELWRHIGPETDVPAGDIGVGGREVGFMFGMYK
KLAHEFTGTFTGKGREFGGSLIRPEATGYGNIYFLLEMLKTKGTDLKGKTCLISGSGNVA
QYTAEKVLEMGGKVLTMSDSDGYIYDPAGIDREKLDYIMELKNLYRGRIREYAEQYPGVK
YVEGARPWGEKADIALPSATQNEINGDDAKKLIANGVMAVSEGANMPSTPEAIKVFQEAK
ILYAPGKAANAGGVSVSGLEMTQNSIKLGWSAEEVDEKLKSIMKNIHEACVQYGTEADGY
VNYVKGANVAGFMKVAKAMMSQGIL