Protein Info for HMPREF1181_RS04250 in Bacteroides stercoris CC31F

Annotation: phenylacetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 91 to 287 (197 residues), 66.2 bits, E=2.4e-22
• PF14535: AMP-binding_C_2
  • amino acids 337 to 432 (96 residues), 96.1 bits, E=1.2e-31

Best Hits

Swiss-Prot: 45% identical to PAAK_THET2: Phenylacetate-coenzyme A ligase (TT_C0602) from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 90% identity to bfr:BF1693)

MetaCyc: 45% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

• penicillin G and penicillin V biosynthesis (1/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (2/9 steps found)
• phenylacetate degradation I (aerobic) (1/9 steps found)
• superpathway of phenylethylamine degradation (1/11 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (2/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Protein Sequence (435 amino acids)

>HMPREF1181_RS04250 phenylacetate--CoA ligase (Bacteroides stercoris CC31F)
MNDKYWEEEIETMPREELHKLQLRRLRKTIGIAANSPYYKQVFQKHGITADSIRSIEDIR
KIPFTTKADMRANYPFGLVAGNMHEDGVRIHSSSGTTGTPTVIVHSRHDLESWTNLVARC
LYAVGIRKTDVFQNSSGYGMFTGGLGFQYGAERLGALTVPAAAGNSKRQIKFITDFRTTA
LHAIPSYAIRLAEVFQEEGIDPASTTLKTLVIGAEPHTDEQRRKIERILGVKAYNSFGMT
EMNGPGVAFECTEQNGMHLWEDCYLVEIINPETGEPVPDGEIGELVLTTLDREMMPLIRY
RTRDLTRILPDTCPCGRTHLRIDRIKGRSDDMFIIKGVNIFPMQVEKVLVQFPELGSNYL
ITLETISNQDEMIMEVELSDLSTDNYIELEKVRKAISRRLKDEILVTPKVKLVKKGSLPQ
SEGKAVRVKDLRDNK