Protein Info for HMPREF1181_RS03320 in Bacteroides stercoris CC31F

Annotation: glutamate-5-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 72% identical to PROA_BACFN: Gamma-glutamyl phosphate reductase (proA) from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 76% identity to bhl:Bache_0329)

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
L-ornithine biosynthesis II (2/3 steps found)
L-citrulline biosynthesis (5/8 steps found)
L-N^δ-acetylornithine biosynthesis (4/7 steps found)
superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (415 amino acids)

>HMPREF1181_RS03320 glutamate-5-semialdehyde dehydrogenase (Bacteroides stercoris CC31F)
MDLNKTFSAVQYASRKLLALNDEKINQILLAVADAAEKRTDFILAENRKDLDRMDISNPK
YDRLKLTADRLKGIADDIRKVAKLPSPVGRVQKQAVMPNGLKIKRVSVPFGVIGIIYEAR
PNVSFDVFSLCLKSGNACILKGGSDADASNRAIISVIHDVLKQFEVDLHVVELLPSDREA
TAALLHANGYVDLIIPRGSSNLINFVRQNATIPVIETGAGICHTFFDRYGDVKKGAAIVN
NAKTRRVSVCNALDCLLVDGDRMKDLPTLCAPLQESNVIIYADTFAYASLQGHYPAELLK
EATEEDFGTEFLDYKLAVKVVEGMDSAIAHIQQYGSKHSECIVTEDKGHADWFCREVDAA
CVYVNAPTSFTDGAQFGLGAEIGISTQKLHARGPMALEEITTYKWIIEGEGQIRK