Protein Info for HMPREF1181_RS01170 in Bacteroides stercoris CC31F

Annotation: phosphoenolpyruvate carboxykinase (ATP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00224: phosphoenolpyruvate carboxykinase (ATP)
  • amino acids 5 to 535 (531 residues), 826.6 bits, E=5.2e-253
• PF01293: PEPCK_ATP
  • amino acids 16 to 485 (470 residues), 666 bits, E=1.5e-204

Best Hits

Swiss-Prot: 94% identical to PCKA_BACFN: Phosphoenolpyruvate carboxykinase (ATP) (pckA) from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 97% identity to bhl:Bache_0916)

MetaCyc: 70% identical to phosphoenolpyruvate carboxykinase (ATP) (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate carboxykinase (ATP). [EC: 4.1.1.49]

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

• gluconeogenesis I (11/13 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (6/14 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.49

Protein Sequence (535 amino acids)

>HMPREF1181_RS01170 phosphoenolpyruvate carboxykinase (ATP) (Bacteroides stercoris CC31F)
MANLDLSKYGIKDVKEIIHNPSYDVLFAEETKPGLEGFEKGQVTELGAVNVMTGIYTGRS
PKDKFFVMNEASKDSVWWTSEEYKNDNKPCSEEAWADLKAKAVNQLSGKRLFVVDTFCGA
NEATRMKVRFIMEVAWQAHFVTNMFIRPTAEELANYGEPDFVSFNASKAKVDNYKELGLN
SETATVFNLKTNEQVILNTWYGGEMKKGMFSIMNYKNPLRGIASMHCSANTNMEGTDSAI
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDDEGVFNYEGGCYAKVINLDKESEPDIYNAIK
RDALLENVTVDADGKIDFADKSTTENTRVSYPIYHINNIVKPVSKGPHAHQVIFLSADAF
GVLPPVSILNPEQAQYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYAEEL
VKKMNKVGAKAYLVNTGWNGSGKRISIKDTRGIIDAILDGSIDKAPTKVIPYFDFVVPTE
LPGVDPKILDPRDTYECACQWEEKAKDLAGRFIKNFAKFTGNEAGKALVAAGPKL