Protein Info for HMPREF1181_RS01100 in Bacteroides stercoris CC31F

Annotation: arginine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF03485: Arg_tRNA_synt_N" amino acids 10 to 88 (79 residues), 23.8 bits, see alignment E=8.8e-09 TIGR00456: arginine--tRNA ligase" amino acids 25 to 605 (581 residues), 430.1 bits, see alignment E=5.7e-133 PF00750: tRNA-synt_1d" amino acids 110 to 470 (361 residues), 162.3 bits, see alignment E=2.4e-51 PF05746: DALR_1" amino acids 486 to 605 (120 residues), 104.9 bits, see alignment E=4.3e-34

Best Hits

Swiss-Prot: 87% identical to SYR_BACFN: Arginine--tRNA ligase (argS) from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 92% identity to bhl:Bache_0930)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (605 amino acids)

>HMPREF1181_RS01100 arginine--tRNA ligase (Bacteroides stercoris CC31F)
MNIEQKLVASVMSGLKALYGQDVPAAQIQLQKTKKEFEGHLTLVVFPFLRMSKKGPEQTA
QEIGEYLQANEPSVSAFNVIKGFLNLTIASSAWIELLNGIHADAKYGIVAATDNAPLVMI
EYSSPNTNKPLHLGHVRNNLLGNALANIISANGNRVVKTNIVNDRGIHICKSMLAWQKYG
NGETPESSGKKGDHLIGDYYVAFDKHYKAEVKELMAKFQSEGMSEEEAKAKAEAESPLMK
EAREMLVKWEAGDPEVRTLWKKMNDWVYAGFDETYRMMGVTFDKIYYESETYLEGKEKVM
EGLEKGFFYRKEDGSVWANLTGEGLDHKLLLRADGTSVYMTQDIGTAKLRFADFPIDKMV
YVVGNEQNYHFQVLSILLDKLGFEWGKGLVHFSYGMVELPEGKMKSREGTVVDADDLMAE
MINTAKETSGELGKLDGLTQEEADNIARIVGLGALKYFILKVDARKNMTFNPKESIDFNG
NTGPFIQYTYARIQSVLRKAKEAGISVPAQLPAGIELSEKEEGLIQMVADFAAIVKQAGE
DYSPSLIANYTYDLVKEYNQFYHDFSILREENEAVKIFRLALSENVAKVVHLGMGLLGIE
VPDRM