Protein Info for HGI48_RS19710 in Dickeya dianthicola 67-19

Annotation: peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 852 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00912: Transgly" amino acids 54 to 229 (176 residues), 227 bits, see alignment E=1.7e-71 TIGR02074: penicillin-binding protein, 1A family" amino acids 65 to 783 (719 residues), 575 bits, see alignment E=8.6e-177 PF17092: PCB_OB" amino acids 316 to 425 (110 residues), 109.7 bits, see alignment E=2.1e-35 PF00905: Transpeptidase" amino acids 429 to 732 (304 residues), 86.5 bits, see alignment E=2.9e-28

Best Hits

Swiss-Prot: 76% identical to PBPA_ECOLI: Penicillin-binding protein 1A (mrcA) from Escherichia coli (strain K12)

KEGG orthology group: K05366, penicillin-binding protein 1A [EC: 2.4.1.- 3.4.-.-] (inferred from 98% identity to ddd:Dda3937_00363)

MetaCyc: 76% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcA (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-, 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.- or 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RI28 at UniProt or InterPro

Protein Sequence (852 amino acids)

>HGI48_RS19710 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA (Dickeya dianthicola 67-19)
MKFVKYLFILAVSCILLGAASLYGLYRYIEPQLPDVATLKDVRLQTPMQVYSADGELIAQ
FGEKRRIPLKLDQIPPTLVNAFIATEDSRFFEHHGVDPQGIIRAAMIALTSGHASQGAST
ITQQLARNFFLTPERTLLRKIKEVFLAIRIEQTLTKEEILELYLNKIYLGYRAYGVGAAA
QVYFGRPVEQLTLAQMAMIAGLPKAPSTFNPLYSYDRAVARRNVVLSRMLDENYITQAQY
DQARNEKLVADYHAPEIAFSASYLAEMVRQEMVKRYGEDAYNDGYQVYTTITRKLQQAAE
DAVHNNVIAYDMRHGYRGPEQVLWKVGAPALAQDKIVEALKKLPVYGPLFPAVVTDASAD
KAHAMMANGDKIDLPLAGVRWARAYRSDDQQGPTPKRVTDVLEAGQQIWVRKVDNDWWLA
QVPDVNSALVSLNPKDGAVLALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGL
TLATILNDAPITRWDPGAGSDWSPKNSPPEYDGQIRLRQGLGQSKNVVMVRAMRAMGVDY
AADYLERFGFPEQNIVHTESLALGSASFTPLQVVRGYAVMTNGGYLVDPYFITKIDNASG
NTVFTAIPKIVCDTCNLPVIYGDTQRSAVLSDDSMENVATSQENPVQSAPQADPATPSEV
ARQAATQPYAPHVISTPLSFLIKDALNSNVFGEPGWMGTGWRAGKVLQRRDIGGKTGTTN
NSKDAWFSGYGPNIVASVWIGFDDHRRDLGRTSASGVIRDQISGYEGGAKSAEPAWDDFM
KVALAGVPEQKTPPPQGVVTVTIDRSSGKLSDGGANSRQEFFIDGTQPTERAVHDAGTTL
FNTSSGQAEELF