Protein Info for HGI48_RS19150 in Dickeya dianthicola 67-19

Annotation: malate synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 10 to 70 (61 residues), 71.8 bits, E=3.9e-24
• TIGR01344: malate synthase A
  • amino acids 23 to 531 (509 residues), 827.7 bits, E=1.4e-253
• PF01274: MS_TIM-barrel
  • amino acids 161 to 406 (246 residues), 380.3 bits, E=4.5e-118
• PF20659: MS_C
  • amino acids 411 to 530 (120 residues), 148.7 bits, E=1.4e-47

Best Hits

Swiss-Prot: 81% identical to MASY_ECOLI: Malate synthase A (aceB) from Escherichia coli (strain K12)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 94% identity to ddd:Dda3937_02127)

MetaCyc: 81% identical to malate synthase A (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• chitin deacetylation (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• L-arabinose degradation IV (3/8 steps found)
• D-xylose degradation IV (2/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (1/14 steps found)
• superpathway of pentose and pentitol degradation (12/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See A0A7L9RI88 at UniProt or InterPro

Protein Sequence (532 amino acids)

>HGI48_RS19150 malate synthase A (Dickeya dianthicola 67-19)
MTQQTISRELVFTTAFGEEERQILTTEAVDFLTELVSRFTPQRNRLLAERQAVQVRIDNG
ELPDFISETDSIRKSNWKIRGIPADLLDRRVEITGPVERKMVINALNANVKVFMADFEDS
LAPSWTKVIEGHINLRDAVRGTITYTSEAGKIYQIKPDPAVLICRVRGLHLPEKHVLWND
EPIPGSLFDFALYVFHNYRQLLAKGSGPYFYLPKTQSWQEAAWWGDVFSYTEDHFGLPRG
TIKATILIETLPAVFQMDEILYHLRDHIVGLNCGRWDYIFSYIKTLKNHPDRVLPDRQSV
TMDKPFLSAYSRLLIKTCHRRGAFAMGGMAAFIPSKDAERNAWVQEKVRQDKELEARNGH
DGTWVAHPGLADTVMPVFDSLLGERPNQLDVLREDDAPVTADELLTPCPGERTEAGMRAN
IRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIRHGKTLSDGRMVTKALFRQ
MLAEEMQVIRHELGDARFDGGRFEEAARLMERITTQDALIDFLTLPGYDLLD