Protein Info for HGI48_RS18785 in Dickeya dianthicola 67-19

Annotation: nif-specific transcriptional activator NifA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 TIGR01817: Nif-specific regulatory protein" amino acids 18 to 524 (507 residues), 695.8 bits, see alignment E=2.2e-213 PF13185: GAF_2" amino acids 34 to 174 (141 residues), 41.4 bits, see alignment E=6e-14 PF01590: GAF" amino acids 36 to 174 (139 residues), 37.8 bits, see alignment E=9.3e-13 PF00158: Sigma54_activat" amino acids 209 to 375 (167 residues), 244.9 bits, see alignment E=1.2e-76 PF14532: Sigma54_activ_2" amino acids 210 to 380 (171 residues), 71.1 bits, see alignment E=4e-23 PF00004: AAA" amino acids 233 to 358 (126 residues), 22.6 bits, see alignment E=4.5e-08 PF07728: AAA_5" amino acids 233 to 352 (120 residues), 29.6 bits, see alignment E=2.2e-10 PF02954: HTH_8" amino acids 481 to 515 (35 residues), 45.9 bits, see alignment 1.3e-15

Best Hits

Swiss-Prot: 77% identical to NIFA_KLEOX: Nif-specific regulatory protein (nifA) from Klebsiella oxytoca

KEGG orthology group: K02584, Nif-specific regulatory protein (inferred from 95% identity to ddd:Dda3937_02180)

Predicted SEED Role

"Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RHM8 at UniProt or InterPro

Protein Sequence (524 amino acids)

>HGI48_RS18785 nif-specific transcriptional activator NifA (Dickeya dianthicola 67-19)
MTQAPDTSHVVWRFDLSQQFTAVQRISAALSRASDVGQALQAVLRVLHDDAFMQHGMICL
YDPQRGALRVEALHSEDQRVSSSSSQVCYRPGEGLVGAVMAQRQPLVLPRVADDQRFLDR
LNLYDYELPFIGVPLLGIEPQPIGVLAAQPMARYEERLPASTRFLETVASLIVQTVRLMQ
APRRDGEPSSVVSRPASCKAPRPFGFDSMVGTSAAMRQTLEIVRQVSRWDTTVLVRGESG
TGKELVANAIHHNSPRAAAPFVKFNCAALPDNLLESELFGHEKGAFTGAVRQRKGRFELA
DGGTLFLDEIGESSASFQAKLLRILQEGEMERVGGDETLRVNVRIIAATNRHLEEEVRAG
HFREDLYYRLNVMPVSLPPLRERQEDIVELAQFLVKKIAAHQGRTLRISDGAIRLLMGYN
WPGNVRELENCLERAAVMTDTGLIDRDVILFNHHDAAPMMNKTTSPPVVAEEGWLDQNLD
ERQRLIAALEKTGWVQAKAARLLGMTPRQVAYRIQIMDINMHRL