Protein Info for HGI48_RS18780 in Dickeya dianthicola 67-19

Annotation: nitrogenase cofactor biosynthesis protein NifB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 TIGR01290: nitrogenase cofactor biosynthesis protein NifB" amino acids 23 to 445 (423 residues), 666.4 bits, see alignment E=9.1e-205 PF04055: Radical_SAM" amino acids 48 to 224 (177 residues), 80.6 bits, see alignment E=1.5e-26 PF02579: Nitro_FeMo-Co" amino acids 348 to 448 (101 residues), 67.9 bits, see alignment E=7.9e-23

Best Hits

Swiss-Prot: 74% identical to NIFB_KLEPN: FeMo cofactor biosynthesis protein NifB (nifB) from Klebsiella pneumoniae

KEGG orthology group: K02585, nitrogen fixation protein NifB (inferred from 96% identity to ddd:Dda3937_02181)

Predicted SEED Role

"Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RJR0 at UniProt or InterPro

Protein Sequence (465 amino acids)

>HGI48_RS18780 nitrogenase cofactor biosynthesis protein NifB (Dickeya dianthicola 67-19)
MTSCPSTSGCRSDQTDRFTPLQASKVAHHPCYSVSGHHHYARMHLAVAPACNLQCHYCNR
KYDCSNESRPGVVSELLTPEQAVAKAHQVAAAIPQLSVVGIAGPGDPLANIARTFRTLTL
LREQLPDLKLCLSTNGLMLPEAVDRLLDVGVDHVTVTVNTVDADIAARIYAWLWLDGDRY
TGREAGAILLERQQEGIRRLTENGVLVKINSVLIPGINDRHLFEVSQQARRWGAFLHNIM
PLIARPEHGTVFGLNGQPEPDAEALAAARAQCGTAMPQMAHCQQCRADAIGMLGEDRSQQ
FRLSSLPAETQPYLPLLHQRAQVHASIASRGESEEPDACLVAVASSGGDVIDCHFGHADR
FQIYSLSAAGVVLVNERFAPKYCRGEDDCEPADSEDRMAAMLALLADVEAVFCARIGYAP
WQKLEQQGIQPCVEGAWQNIADVLGRWWRQRQQARPAGERAQGAA