Protein Info for HGI48_RS17560 in Dickeya dianthicola 67-19

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 467 TIGR00665: replicative DNA helicase" amino acids 22 to 460 (439 residues), 584.7 bits, see alignment E=5.6e-180 PF00772: DnaB" amino acids 23 to 124 (102 residues), 125.8 bits, see alignment E=1e-40 PF03796: DnaB_C" amino acids 202 to 458 (257 residues), 364.1 bits, see alignment E=5.7e-113 PF13481: AAA_25" amino acids 218 to 381 (164 residues), 39.4 bits, see alignment E=7.9e-14

Best Hits

Swiss-Prot: 88% identical to DNAB_SALTI: Replicative DNA helicase (dnaB) from Salmonella typhi

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 98% identity to ddc:Dd586_3433)

MetaCyc: 84% identical to replicative DNA helicase (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RGX3 at UniProt or InterPro

Protein Sequence (467 amino acids)

>HGI48_RS17560 replicative DNA helicase (Dickeya dianthicola 67-19)
MAEKKPTKSAEPRDRQVEGLKLPPHSIEAEQSVLGGLMLDNERWDNVAERVSANDFFNRA
HRLIFNEMQRLLEMSKPIDLITLSESLEQKGDLESAGGFAYLAELAKNTPSAANIGAYAD
IVRERAVVREMIAVANEIADAGYDPQGRSSEELLDLAESRVFQIAENRASKDEGPKSIDR
ILEDTVSRIEQLYQRPHDGVTGVSSGYQDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNL
CENAAMTQEKPVLIFSLEMPGEQLMMRMLASLSRVDQTRIRTGQLDDEDWARISSTMGLL
LEKRNMYIDDSSGLTPTDVRSRARRVFREHDGLSLIMIDYLQLMRVPSLSDNRTLEIAEI
SRSLKALAKELQVPVIALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVY
HENSDLKGIAEIIIGKQRNGPIGSVRLTFNGQWSRFDNYAGPQYDDE