Protein Info for HGI48_RS17070 in Dickeya dianthicola 67-19

Annotation: GTP diphosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 745 PF13328: HD_4" amino acids 48 to 191 (144 residues), 149.4 bits, see alignment E=2.2e-47 TIGR00691: RelA/SpoT family protein" amino acids 63 to 740 (678 residues), 624.3 bits, see alignment E=1.3e-191 PF04607: RelA_SpoT" amino acids 251 to 360 (110 residues), 144.8 bits, see alignment E=3.5e-46 PF02824: TGS" amino acids 405 to 464 (60 residues), 76 bits, see alignment 5.6e-25 PF19296: RelA_AH_RIS" amino acids 483 to 646 (164 residues), 46.2 bits, see alignment E=1.9e-15 PF13291: ACT_4" amino acids 665 to 741 (77 residues), 65.3 bits, see alignment E=2e-21 PF01842: ACT" amino acids 669 to 734 (66 residues), 39.2 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 85% identical to RELA_ECOL6: GTP pyrophosphokinase (relA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 99% identity to ddd:Dda3937_00195)

MetaCyc: 85% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RGQ6 at UniProt or InterPro

Protein Sequence (745 amino acids)

>HGI48_RS17070 GTP diphosphokinase (Dickeya dianthicola 67-19)
MVAVRSAHLNTEGKFVPDAWIASLGIASKTSCERLAETWRYCEEKTEGHPDAMLLLWRGI
EMVEILSTLSMDNDSMRAAMLFPLADANVVDEETLQETFGKNIVNLVHGVRDMDAIRQLK
ATQHDSMASEQVDNIRRMLLAMVEDFRCVVIKLAERIAHLREVKDAPEEERVLAAKECTN
IYAPLANRLGIGQLKWELEDFCFRYLHPDEYKRIAKLLHERRIDREQYIDDFVKNLRASM
TQEELKAEVYGRPKHIYSIWRKMQKKSLSFDELFDVRAVRIVVERLQDCYAALGIVHTHY
RHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTLEIQIRTRQMHEDAELGVAAHWKYKEG
AATSGRSGYEERIAWLRKLLAWQEEMADSDEMLDEVRSQVFDDRVYVFTPKGDVVDLPIG
STPLDFAYHIHSDIGHRCIGAKVGGRIVPFTYQLQMGDQIEVITQKQPNPSRDWLNPNLG
YITTSRGRSKIHNWFRKQDRDKNILAGKQILDDELAHLGVSLKMAEKLLLPRYNINSMDE
LLAGIGGGDIRLNQLVNFLQSKVNQPSAEEQDREALRQLTQKSHQPAQRASAKDNGRVVV
EGVGNLMHHIARCCQPIPGDDIIGFITRGRGISIHRADCEQLDDLRSHAPERIVEAVWGE
SYSSGYSLVVRVTANDRSGLLRDITTILANEKVNVLGVSSRSDVKQQLATIDMDIEIYNL
QVLGRVLAKLNQLPDVIDARRLQGN