Protein Info for HGI48_RS16995 in Dickeya dianthicola 67-19

Annotation: sulfate adenylyltransferase subunit CysD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02039: sulfate adenylyltransferase, small subunit
  • amino acids 9 to 302 (294 residues), 556.1 bits, E=1.1e-171
• PF01507: PAPS_reduct
  • amino acids 29 to 256 (228 residues), 202.6 bits, E=2.5e-64

Best Hits

Swiss-Prot: 95% identical to CYSD_KLEP7: Sulfate adenylyltransferase subunit 2 (cysD) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00957, sulfate adenylyltransferase subunit 2 [EC: 2.7.7.4] (inferred from 99% identity to ddd:Dda3937_00213)

MetaCyc: 94% identical to sulfate adenylyltransferase subunit 2 (Escherichia coli K-12 substr. MG1655)
Sulfate adenylyltransferase. [EC: 2.7.7.4]

Predicted SEED Role

"Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Sequence Analysis Tools

See A0A3A4C9D3 at UniProt or InterPro

Protein Sequence (302 amino acids)

>HGI48_RS16995 sulfate adenylyltransferase subunit CysD (Dickeya dianthicola 67-19)
MDEKRLTHLKQLEAESIHIIREVAAEFNNPVMLYSIGKDSSVMLHLARKAFFPGTLPFPL
LHVDTGWKFREMYQFRDRTAKAYGCELLVHHNPEGVAMGINPFVHGSAKHTDIMKTEGLK
QALDKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWNNYNGQINKGES
IRVFPLSNWTELDIWQYIYLENIDIVPLYLAAERPVLERDGMLLMVDDDRIDLQPGEVIA
QRMVRFRTLGCWPLTGAVESQAQTLPEIIEEMLVSTTSERQGRVIDRDQAGSMELKKRQG
YF