Protein Info for HGI48_RS16640 in Dickeya dianthicola 67-19

Annotation: beta-Ala-His dipeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 TIGR01893: Xaa-His dipeptidase" amino acids 8 to 482 (475 residues), 587.3 bits, see alignment E=1.1e-180 PF01546: Peptidase_M20" amino acids 73 to 480 (408 residues), 57.7 bits, see alignment E=1.6e-19 PF07687: M20_dimer" amino acids 209 to 292 (84 residues), 37.3 bits, see alignment E=2.3e-13

Best Hits

Swiss-Prot: 79% identical to PEPD_ECOLI: Cytosol non-specific dipeptidase (pepD) from Escherichia coli (strain K12)

KEGG orthology group: K01270, aminoacylhistidine dipeptidase [EC: 3.4.13.3] (inferred from 96% identity to ddd:Dda3937_00930)

MetaCyc: 79% identical to peptidase D (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]

Predicted SEED Role

"Aminoacyl-histidine dipeptidase (Peptidase D) (EC 3.4.13.3)" (EC 3.4.13.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.13.18 or 3.4.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RGW1 at UniProt or InterPro

Protein Sequence (486 amino acids)

>HGI48_RS16640 beta-Ala-His dipeptidase (Dickeya dianthicola 67-19)
MSALSQLSPQPLWDIFAKICSIPHPSYHEEALAAYILSRAQERGLHTQRDQVGNILLRKP
ATPGMENRKPVVIQAHLDMVPQKNNDTVHDFTTDPIQPYIDGEWIKARGTTLGADNGIGM
ASALAVLFDDGVEHGPLEVLLTMTEEAGMDGAFGLQPNWLQGDILINTDSEEEGEIYMGC
AGGIDFLTRLPLQREAVPTGYQTLKLVIKGLKGGHSGCDIHLGLGNVNKLLARFLAQHAA
ELDLRVIDLHGGTLRNAIPREGAITLAVSAEKADALKAAAQAYLAVLKHELAAVEKNLTL
LLEAADSTTAALTAASRDRFIALLNSTPNGVIRMSDDVKGVVETSLNVGVVGMTETQAEI
HCLIRSLVDSGKDYVVSMLTSLGQLAGADTLAKGGYPGWQPDAQSPVMQLVRETYQTLFG
KTPNIMVIHAGLECGLFKKPYPEMDMVSIGPTITGPHSPDEQVHIASVGQYWKLLTALLK
AIPERA