Protein Info for HGI48_RS16380 in Dickeya dianthicola 67-19

Annotation: (S)-acetoin forming diacetyl reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00106: adh_short" amino acids 4 to 197 (194 residues), 209.2 bits, see alignment E=6.3e-66 TIGR02415: acetoin reductases" amino acids 4 to 257 (254 residues), 404.1 bits, see alignment E=1.1e-125 PF08659: KR" amino acids 6 to 165 (160 residues), 47 bits, see alignment E=4.4e-16 PF13561: adh_short_C2" amino acids 10 to 254 (245 residues), 193.8 bits, see alignment E=5.5e-61

Best Hits

Swiss-Prot: 85% identical to BUDC_KLEPN: Diacetyl reductase [(S)-acetoin forming] (budC) from Klebsiella pneumoniae

KEGG orthology group: K03366, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 99% identity to ddd:Dda3937_03162)

MetaCyc: 85% identical to acetoin reductase subunit (Klebsiella pneumoniae)
RXN-11032 [EC: 1.1.1.304]; 1.1.1.- [EC: 1.1.1.304]

Predicted SEED Role

"2,3-butanediol dehydrogenase, S-alcohol forming, (R)-acetoin-specific (EC 1.1.1.4) / Acetoin (diacetyl) reductase (EC 1.1.1.5)" in subsystem Acetoin, butanediol metabolism (EC 1.1.1.4, EC 1.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.303 or 1.1.1.304 or 1.1.1.4 or 1.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RGE1 at UniProt or InterPro

Protein Sequence (257 amino acids)

>HGI48_RS16380 (S)-acetoin forming diacetyl reductase (Dickeya dianthicola 67-19)
MKQKVALVTGAGQGIGKAIALRLAKDGFAVAVVDYNSDTAGQVAQEIRHHGGNAIALTAD
VSNRDQVFDAVRTAHKQLGGFHVIVNNAGIAPTTLIEDITPEIVDKVYNINVKGVIWGIQ
AAVEAFKAQSQGGKIINAASQAGHVGNPELAVYSSSKFAVRGLTQTAARDLAPLGITVNA
YCPGIVKTPMWEEIDRQISEAAGKPLGYGTAEFAKRITLGRLSEPEDVAACVSYLAGPDS
DYMTGQALLIDGGMVFN