Protein Info for HGI48_RS16345 in Dickeya dianthicola 67-19

Annotation: alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00344: alanine--tRNA ligase
  • amino acids 7 to 854 (848 residues), 1134.1 bits, E=0
• PF01411: tRNA-synt_2c
  • amino acids 7 to 554 (548 residues), 770.9 bits, E=1.1e-235
• PF07973: tRNA_SAD
  • amino acids 653 to 696 (44 residues), 62.9 bits, 3.4e-21
• PF02272: DHHA1
  • amino acids 732 to 871 (140 residues), 112 bits, E=4.9e-36

Best Hits

Swiss-Prot: 81% identical to SYA_CROS8: Alanine--tRNA ligase (alaS) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 98% identity to ddd:Dda3937_03152)

MetaCyc: 81% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• tRNA charging (21/21 steps found)
• enterobactin biosynthesis (7/11 steps found)
• bacillibactin biosynthesis (6/12 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (3/8 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (2/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (3/30 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

See A0A7L9RGA5 at UniProt or InterPro

Protein Sequence (875 amino acids)

>HGI48_RS16345 alanine--tRNA ligase (Dickeya dianthicola 67-19)
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGQDKRNYV
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIIYAWELLTSPQW
FGLPKEKLWVTVYATDDEAYDIWANEVGVPHERIIRIGDNKGAPYASDNFWQMGDTGPCG
PCSEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNLVFMQFNRQADGTMLPLPKPSVDTGMG
LERISAVLQHVNSNYEIDLFNTLIAEVASVVGTDDLNNKSLRVIADHIRSCAFLIADGVM
PSNENRGYVLRRIIRRAIRHGNMLGAKETFFYKLVGTLIKVMGPAAEELKRQQGLVEQAL
KGEEEQFARTLERGLLLLDEEIKQLQGDTLSGEAAFRLYDTYGFPVDLTADVCRERNLKV
DEAGFERAMDAQRQRARDASGFGADYNSLIRVDESTTFCGYERTEQQSQVVAIYRQGESV
QEVQAGDDAVVVLSETPFYGESGGQVGDQGELTSATARFSVADAQKYGQAIGHIGKLTHG
VLRVNDKVDAVVDHVRRDRIRLNHSATHLLHAALRQVLGEHVAQKGSLVNDSYLRFDFSH
PEAMKPEQIRQVEDIVNTQIRRNLPVETDVMALDAARAKGAMALFGEKYEENVRVLSMGD
FSIELCGGTHARRTGDIGLFRIVSESGTAAGIRRIEAVTGENAIVALHHQNDLLHEVTQL
VKGDSNNLADKIRSLLERTRGLEKELQQLKEQQAAQESSSLSSKAKDINGVKLLVTQLSN
VEPKLLRTMVDDLKNQLGSAVIVLSTVADGKVSLIAGVTKELTDRVKAGELVGFVANQVG
GKGGGRPDMAQAGGSDVDALPSALTSVEAWVVDKL