Protein Info for HGI48_RS15555 in Dickeya dianthicola 67-19

Annotation: ribosome biogenesis GTPase Der

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03594: ribosome-associated GTPase EngA
  • amino acids 3 to 463 (461 residues), 592.6 bits, E=7.4e-182
• TIGR00231: small GTP-binding protein domain
  • amino acids 4 to 158 (155 residues), 78.8 bits, E=6.2e-26
  • amino acids 208 to 371 (164 residues), 81.6 bits, E=8.1e-27
• PF01926: MMR_HSR1
  • amino acids 5 to 119 (115 residues), 103.4 bits, E=3.5e-33
  • amino acids 210 to 327 (118 residues), 97.8 bits, E=2e-31
• PF02421: FeoB_N
  • amino acids 5 to 157 (153 residues), 58.5 bits, E=2.8e-19
  • amino acids 209 to 370 (162 residues), 46.6 bits, E=1.2e-15
• PF00009: GTP_EFTU
  • amino acids 209 to 375 (167 residues), 42.2 bits, E=3.1e-14
• PF14714: KH_dom-like
  • amino acids 383 to 463 (81 residues), 90.1 bits, E=4.1e-29

Best Hits

Swiss-Prot: 86% identical to DER_PECCP: GTPase Der (der) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K03977, GTP-binding protein (inferred from 97% identity to ddd:Dda3937_03709)

MetaCyc: 82% identical to 50S ribosomal subunit stability factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A7L9RFY1 at UniProt or InterPro

Protein Sequence (495 amino acids)

>HGI48_RS15555 ribosome biogenesis GTPase Der (Dickeya dianthicola 67-19)
MIPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGRAEMEGNEFIIIDTGGI
DGNEDGVETRMAEQSLLAIEEADIVLFLVDARDGLMPADHAIAQHLRMREKDTFLVANKI
DGIDIDSGIADFYSLGLGEVHPIAASHGRGVTSLLEKVLLPFAAEPAQETRELTEEEANA
AYWAEQLGEGDDAIDGEDTGPNLEGLPIKLAIVGRPNVGKSTLTNRILGEERVVVYDMPG
TTRDSIYIPMERDGRDYVLIDTAGVRKRGKITDAVEKFSVIKTLQAIEDANVVLLVIDAR
EGISDQDLSLLGFILNSGRSLVIVVNKWDGLTQEVKDQVKETLDLRLGFIDFARIHFISA
LHGSGVGNLFESVTEAYDCSTRRVGTAMLTRIMQMAADDHQPPLVRGRRVKLKYAHAGGY
NPPIVVIHGNQVKDLPDSYKRYLMNYYRRSLEVMGTPIRIQFKEGENPFADKRNTLTPNQ
LRKRKRLMQHIKKGK