Protein Info for HGI48_RS14435 in Dickeya dianthicola 67-19

Annotation: tRNA pseudouridine(38-40) synthase TruA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 14 to 250 (237 residues), 257 bits, see alignment E=8.1e-81 PF01416: PseudoU_synth_1" amino acids 20 to 115 (96 residues), 55.7 bits, see alignment E=3.1e-19 amino acids 156 to 256 (101 residues), 102.7 bits, see alignment E=7.9e-34

Best Hits

Swiss-Prot: 84% identical to TRUA_CROS8: tRNA pseudouridine synthase A (truA) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 98% identity to ddd:Dda3937_02105)

MetaCyc: 80% identical to tRNA pseudouridine38-40 synthase (Escherichia coli K-12 substr. MG1655)
tRNA-pseudouridine synthase I. [EC: 5.4.99.12]

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RFS4 at UniProt or InterPro

Protein Sequence (276 amino acids)

>HGI48_RS14435 tRNA pseudouridine(38-40) synthase TruA (Dickeya dianthicola 67-19)
MTALQHADDEAPLKIALGIEYDGSRYYGWQRQAEVDSVQGCLEAALSQVANERIDVFCAG
RTDAGVHATGQVVHFITRAARKDAAWTMGVNANLPLDIAVRWVKTVDDAFHARFSATARR
YRYVIYNRRFRPAILSRGVTHYYLPLDAERMHRAGQCLLGENDFTSFRAMQCQSRTPWRN
LMHLRVARQGDYVVVDIKANAFVHHMVRNIVGSLMEIGCGNQPENWMAELLAAKDRTLAA
ATAKADGLYLVSVDYPQDFGIPCASMGPLFLPDEAN