Protein Info for HGI48_RS14090 in Dickeya dianthicola 67-19

Annotation: nitrite reductase small subunit NirD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1399 PF07992: Pyr_redox_2" amino acids 4 to 289 (286 residues), 181.1 bits, see alignment E=2.6e-56 amino acids 424 to 721 (298 residues), 186 bits, see alignment E=8.4e-58 PF00070: Pyr_redox" amino acids 144 to 212 (69 residues), 51.2 bits, see alignment 1.1e-16 amino acids 566 to 637 (72 residues), 56.7 bits, see alignment 2.1e-18 PF18267: Rubredoxin_C" amino acids 323 to 389 (67 residues), 47.2 bits, see alignment 1.3e-15 amino acids 743 to 807 (65 residues), 42.4 bits, see alignment 4.2e-14 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 424 to 1222 (799 residues), 1123.4 bits, see alignment E=0 PF04324: Fer2_BFD" amino acids 847 to 894 (48 residues), 41.3 bits, see alignment (E = 1.2e-13) PF03460: NIR_SIR_ferr" amino acids 983 to 1044 (62 residues), 44.4 bits, see alignment (E = 8.7e-15) PF01077: NIR_SIR" amino acids 1055 to 1191 (137 residues), 80.7 bits, see alignment E=6e-26 PF13806: Rieske_2" amino acids 1276 to 1384 (109 residues), 97.2 bits, see alignment 4e-31 TIGR02378: nitrite reductase [NAD(P)H], small subunit" amino acids 1276 to 1384 (109 residues), 86.2 bits, see alignment 1.5e-28

Best Hits

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RF60 at UniProt or InterPro

Protein Sequence (1399 amino acids)

>HGI48_RS14090 nitrite reductase small subunit NirD (Dickeya dianthicola 67-19)
MSAHLVIIGNGLSSARLVKRLGQLAPQRYRVTVIDREPRDSYNRVLLSSVLAGEKIFEDT
QLEPAPATMDVTILTGESALSIDRDAREVTTDRRRLAYDQLVFATGSRPFMPPLPGIDLP
GVMGFRTLDDVERMRAAVRQGRPVVVIGGGLLGIEAAAALRLQGATVTLLHRHARLMERQ
LDATASDLLCSRLNERGIRCLTGVQIRALSSNEWSGNEWSGNGQVEAVELADGTRLPAGL
VVVATGVQPVCELARDSGLACGRGILADGQLRTADEYISAFGECAEVKGETVGLVAPCLA
QADVLAARLAGRPVADYAPAALATRLKVTGIDVVSAGELHPQEGDRLHSLSDPLGGHYRR
LIFRDDRLCGALLFGHVSDSPQLLAVMEARLSSAPSSLLFGLSFPEVDSRSSAVRISVMS
KPVLVVVGHGMVGHHFLEQLVERSLHQQYHVVVFGEERHPAYDRVHLSEYFAGRSAESLS
MVSDGFFEQHGIELRTGCQVAEIDRQRRCVRDANGQETPYDRLVLATGSYPFVPPMAGND
RPGCLVYRTLDDLDAIADEARESRRGVVVGGGLLGLEAANALRQLGLETHVVEFAPRLMA
VQLDDGGAQLLKRKIEALGLQVHTGKETRAITAGEQARHRMNFADGTVLETDLVLFSAGI
RPRDQLARDAGLETGARGGIVINDSCLTSDDAIFAIGECALWQGQMFGLVAPGYQMARTV
AARLAQMAQQEQQEQLFTGADMSTKLKLLGVDVASIGDAHGHTEGSLSYQWNDEPNQVYK
KIIVSADGKRLLGAVLVGDSSDYSTLLQMKLNDMTLPAHPESLILPALDGAAPKGLGVAA
LPDSAQICSCHNVSKADICAAVDNGCTDLASLKACTKAGTGCGGCVPLLKQVMEYQLQQS
GIEVKKDICEHFAYSRQELYHLIRVNRIRSFDELLTRHGHGLGCEICKPLAGSMLASCWN
DYVLKPEHVPLQDTNDRFLANIQKDGTYSVVPRIPGGEITPQGLIAIGQVAARYHLYTKI
TGGQRVDLFGARLEQLPAIWQELIDAGFETGHAYGKSLRTVKSCVGSTWCRYGVQDSTAL
AIALEHRYKGLRSPHKLKMAVSGCTRECAEAQGKDIGVIATDKGWNLYVCGNGGMKPRHA
DLFASDLDTDTLFRLIDRLLMFYIRTGDRLQRTSVWLDNLEGGIDYLRQVVIDDSLGIAD
ELESEMRHVIDSYQCEWQTTLAHDEHRALFRAFLNSDTPDEAVVMVPERGQPRPARLEEK
KPTTATVSDAPGAEGWQCVGFLSDIPAHAGMAARVGHQQVALFHLPAAQPGQAAHVFALD
NQEPGSGANVLSRGIIGDVAGEPVVISPLYKKRLRLRDGVSPDDSQLRVRAWPVRLVQDR
IWVANEPMAVVAADMAEVL