Protein Info for HGI48_RS10320 in Dickeya dianthicola 67-19

Annotation: exodeoxyribonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 TIGR00633: exodeoxyribonuclease III (xth)" amino acids 1 to 266 (266 residues), 296.5 bits, see alignment E=1.7e-92 TIGR00195: exodeoxyribonuclease III" amino acids 1 to 266 (266 residues), 353.8 bits, see alignment E=5.9e-110 PF03372: Exo_endo_phos" amino acids 4 to 259 (256 residues), 131.6 bits, see alignment E=1.7e-42

Best Hits

Swiss-Prot: 80% identical to EX3_SALTI: Exodeoxyribonuclease III (xthA) from Salmonella typhi

KEGG orthology group: K01142, exodeoxyribonuclease III [EC: 3.1.11.2] (inferred from 96% identity to ddc:Dd586_2153)

MetaCyc: 79% identical to exodeoxyribonuclease III (Escherichia coli K-12 substr. MG1655)
3.1.4.-; 3.1.3.-; Deoxyribonuclease IV (phage-T(4)-induced). [EC: 3.1.21.2]; Exodeoxyribonuclease III. [EC: 3.1.21.2, 3.1.11.2]

Predicted SEED Role

"Exodeoxyribonuclease III (EC 3.1.11.2)" in subsystem DNA repair, bacterial (EC 3.1.11.2)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.2

Use Curated BLAST to search for 3.1.11.2 or 3.1.21.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RDP8 at UniProt or InterPro

Protein Sequence (268 amino acids)

>HGI48_RS10320 exodeoxyribonuclease III (Dickeya dianthicola 67-19)
MKFVSFNINGLRARPHQLAAIIAQHQPDVIGLQETKVHDDMFPLEEVKQFGYHVFYHGQK
GHYGVALLTKAEPLAIRRGFPTDEEDAQRRIIMADLATPLGTLTVVNGYFPQGESRDHPV
KFPAKTRFYQDLQHYLDQHHQADQPVLIMGDMNISPTDLDIGIGEDNRKRWLRTGKCSFL
PEEREWMERLKNWGLVDTFRAANPECQDRFSWFDYRSAGFDDNRGLRIDLIMATAPLASR
CAATGIDYAIRGMEKPSDHAPVWAEFTL