Protein Info for HGI48_RS07940 in Dickeya dianthicola 67-19

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 TIGR01891: amidohydrolase" amino acids 12 to 368 (357 residues), 347.4 bits, see alignment E=5e-108 PF01546: Peptidase_M20" amino acids 69 to 380 (312 residues), 151.5 bits, see alignment E=3.1e-48 PF07687: M20_dimer" amino acids 180 to 272 (93 residues), 47.8 bits, see alignment E=1.3e-16

Best Hits

Swiss-Prot: 39% identical to ILL7_ORYSJ: IAA-amino acid hydrolase ILR1-like 7 (ILL7) from Oryza sativa subsp. japonica

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 95% identity to ddd:Dda3937_00819)

MetaCyc: 47% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"Metal-dependent amidase/aminoacylase/carboxypeptidase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RC69 at UniProt or InterPro

Protein Sequence (385 amino acids)

>HGI48_RS07940 amidohydrolase (Dickeya dianthicola 67-19)
MGISPALIAQAVEWRRKLHANPELGYHERQTSGMVATLLAAFGFQVHTGLADTGVVGTLE
NGPGPVIGLRADMDALPVTELSDLTYKSRHSGVMHACGHDGHTAMLLAAASHLSETRRFS
GTVRVVFQPAEENLGGARRMVEEGLFTRFPMDDIYALHNWPGLPVGHVGLSSGAMMASLD
SFEIILTGKSCHAAMPENGADPIMAASQLVLSLQTIPSRRLSPQSSAVISITQIAGGEAI
NVIPEKVVLRGTLRCLQASVREQVKALMAQFVVQIPDVFGVTGSIAYYPGYPVTQNDARA
AQAVREVAEATFGANQVHWDIAPSMASEDFACMLEHRPGAYFWLGADGDTPSRPLHNAGY
DFNDALIPQGVAMWVALVEKRLPIR