Protein Info for HGI48_RS03500 in Dickeya dianthicola 67-19

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 transmembrane" amino acids 7 to 29 (23 residues), see Phobius details PF19335: HMBD" amino acids 50 to 77 (28 residues), 43.4 bits, see alignment (E = 5.1e-15) PF16576: HlyD_D23" amino acids 113 to 321 (209 residues), 212 bits, see alignment E=1.3e-66 PF16572: HlyD_D4" amino acids 158 to 209 (52 residues), 74.1 bits, see alignment 1.3e-24 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 206 to 398 (193 residues), 118.9 bits, see alignment E=1.1e-38 PF13437: HlyD_3" amino acids 217 to 318 (102 residues), 51 bits, see alignment E=4.2e-17

Best Hits

Swiss-Prot: 93% identical to SILB_SALTM: Putative membrane fusion protein SilB (silB) from Salmonella typhimurium

KEGG orthology group: K07798, Cu(I)/Ag(I) efflux system membrane protein CusB (inferred from 91% identity to spc:Sputcn32_0261)

MetaCyc: 66% identical to copper/silver export system membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-90; TRANS-RXN0-280

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RA78 at UniProt or InterPro

Protein Sequence (426 amino acids)

>HGI48_RS03500 efflux RND transporter periplasmic adaptor subunit (Dickeya dianthicola 67-19)
MASLKIKYAAIIISSLIAGGLISVTAWQYLNSVQKTEKTEKTAPERKVLFWYDPMKPDTK
FDKPGKSPFMDMDLIPKYADENGEKSSDGIRIDPTQVQNLGLKTQKVTRGTLNYSQTIPA
NVSYNEYQFVIVQARSDGFVEKVYPLTIGDHVKKGTPLIDITIPEWVEAQSEFLLLSGTG
GTSTQIKGVLERLRLAGMPEEDIQRLRSTRSIQTRFTIKAPIDGVITAFDLRTGMNISKD
KVVAQIQGMDPVWISAAVPESIAYLLKDTSQFEISVPAYPDKTFHVEKWNILPSVDQTTR
TLQVRLQVTNKDELLKPGMNAYLKLNTQSQEMLLIPSQAVIDTGKEQRVITVDDAGRFVP
KMINVLHESQQQSGIGTGLIEGDTVVVSGLFLIDSEANITGALERMRHPETMPNMPTQPA
NAHSGH