Protein Info for HGI48_RS03115 in Dickeya dianthicola 67-19

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 TIGR01391: DNA primase" amino acids 4 to 419 (416 residues), 495.4 bits, see alignment E=6.3e-153 PF01807: zf-CHC2" amino acids 7 to 100 (94 residues), 137.2 bits, see alignment E=5.4e-44 PF08275: DNAG_N" amino acids 126 to 251 (126 residues), 144.7 bits, see alignment E=6.5e-46 PF13662: Toprim_4" amino acids 260 to 327 (68 residues), 55.1 bits, see alignment E=3e-18 PF01751: Toprim" amino acids 261 to 337 (77 residues), 57 bits, see alignment E=7.3e-19 PF13362: Toprim_3" amino acids 261 to 356 (96 residues), 30.5 bits, see alignment E=1.6e-10 PF13155: Toprim_2" amino acids 263 to 349 (87 residues), 65.1 bits, see alignment E=2.4e-21 PF10410: DnaB_bind" amino acids 369 to 424 (56 residues), 54.4 bits, see alignment 4.9e-18 PF08278: DnaG_DnaB_bind" amino acids 451 to 576 (126 residues), 108.1 bits, see alignment E=1.6e-34

Best Hits

Swiss-Prot: 74% identical to DNAG_ECOL6: DNA primase (dnaG) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 98% identity to ddd:Dda3937_03533)

MetaCyc: 74% identical to DNA primase (Escherichia coli K-12 substr. MG1655)
RXN0-5021 [EC: 2.7.7.101, 2.7.7.102]

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.- or 2.7.7.101 or 2.7.7.102

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9RA08 at UniProt or InterPro

Protein Sequence (584 amino acids)

>HGI48_RS03115 DNA primase (Dickeya dianthicola 67-19)
MAGRIPRVFINDLLARTDIIDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGDKQFYH
CFGCGAHGNAIDFLMNYDRLEFVESIEELAAMHSLDVPYEAGTGPTQLERHQRQSLYELM
EQLSAFYQHTLNQPAGTPAREYLSRRGLSDEVIRQFAIGCTPPGWDNALKRFGRSSENRT
TLTDAGMLVTNENGRTYDRFRDRVMFPIRDKRGRVIAFGGRVMGEGMPKYLNSPETEIFH
KGRQLYGLYEAQQKNPELKRLLVVEGYMDVVALAQFGIDYAVASLGTSTTADHIQLLFRA
TDQVVCCYDGDRAGRDAAWRALETALPYLDDGRQLRFMFLPDGEDPDTLVRQEGKAAFEQ
RIEQTMPLSAFLFDSLLQQVDMSTPDGRTKLSTLALPLIGQVPGETLRLYLRQQLGNKLG
LLDDSQLDRLLPKAAEQTWSYQPPRLKVTTMRILIGLLVQNPRLSFEVPELALDGVEENK
VAGLELFLDLVKTCNESPGMNMGLLLEKYRESKYRKQLETLAAWDHMIEEEELEEKFRVS
LTELYDQLLQLRMDTLIARERTHGLSTNERKELWSLQLALTRKN