Protein Info for HGI48_RS01955 in Dickeya dianthicola 67-19

Annotation: leucyl aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 PF02789: Peptidase_M17_N" amino acids 19 to 147 (129 residues), 132.5 bits, see alignment E=8.6e-43 PF00883: Peptidase_M17" amino acids 186 to 488 (303 residues), 418.8 bits, see alignment E=1.4e-129

Best Hits

Swiss-Prot: 94% identical to AMPA_PECCP: Probable cytosol aminopeptidase (pepA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 99% identity to ddc:Dd586_0391)

MetaCyc: 89% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.9

Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A7L9R957 at UniProt or InterPro

Protein Sequence (502 amino acids)

>HGI48_RS01955 leucyl aminopeptidase (Dickeya dianthicola 67-19)
MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKVGQSL
LLHHVPHILSERILLIGCGKERELDERQYKQVVQKTINALNETGSMEAVCFLTELHVKGR
NTYWKVRQAVETAKETLYTFDQLKTNKVELRRPLRKMVFNVPTRRELTSGERAIQHGLAI
ASGIKAAKDLGNMPPNICNAAYLASQARQLADGYSQVTTRVIGEQQMKELGMNAYLAVGQ
GSQNESLMSVIEYKGDPDSNSKPIVLVGKGLTFDAGGISIKPADSMDEMKYDMCGAASVY
GVMRVAAELALPLNIIGILAGCENMVDGRSYRPGDILTTMSGQTVEVLNTDAEGRLVLCD
TLTYVERFEPDVVIDVATLTGACVIALGHHLTGLMSNHNPLAHELLGASEQAGDRAWRLP
LGDEYQEQLDSNFADMANIGGRPGGAITAACFLSRFTRKYSWAHLDIAGTAWRSGKAKGA
TGRPVALLSQFLLNRAGLNDAE