Protein Info for HER17_RS21665 in Pectobacterium carotovorum WPP14

Annotation: glutamine--fructose-6-phosphate transaminase (isomerizing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 610 (609 residues), 868.4 bits, E=1.4e-265
• PF13522: GATase_6
  • amino acids 64 to 184 (121 residues), 67 bits, E=2.7e-22
• PF13537: GATase_7
  • amino acids 81 to 197 (117 residues), 51.9 bits, E=1.1e-17
• PF01380: SIS
  • amino acids 291 to 419 (129 residues), 113.6 bits, E=8.9e-37
  • amino acids 463 to 592 (130 residues), 94.2 bits, E=9e-31

Best Hits

Swiss-Prot: 98% identical to GLMS_PECAS: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 98% identity to pwa:Pecwa_4564)

MetaCyc: 85% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• O-antigen building blocks biosynthesis (E. coli) (8/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (11/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.16

Protein Sequence (610 amino acids)

>HER17_RS21665 glutamine--fructose-6-phosphate transaminase (isomerizing) (Pectobacterium carotovorum WPP14)
MCGIVGAVAQRDVAEILLEGLRRLEYRGYDSAGLAVVDSEGHVARLRRLGKVQVLSQAAE
EHELHGGTGIAHTRWATHGEPSEENAHPHVSEHITIVHNGIIENHEPLRELLIGRGYRFV
SETDTEVVAHLVHFEQQQNGGTLVEIVKRVIPQLRGAYGMVVLDNRDPSVLVAARSGSPL
VIGRGVGENFIASDQLALLPVTRRFMFLEEGDIAEITRRDVRVFDKSGQLAAREEIESKV
NYDAGDKGAYRHYMQKEIYEQPMAIKNTLEGRFSHGEINLSELGPKADELLAKVEHVQII
ACGTSYNSGMVSRYWFEALAGIPCDVEIASEFRYRKPAVRKNSLMITLSQSGETADTLAA
LRLSKELGYLGSLAICNVAGSSLVRESDLALMTKAGVEIGVASTKAFTTQLTVLLMLVAR
VGRLRGMDAKIEHEIVHGLQALPARIEQMLSQDKLIESLAEGFSDKHHALFLGRGDQYPI
AMEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVVVVAPNNELLEKLKSNIEEVRA
RGGELYVFADEDAGFTSSENMKIIPLPHIEEVIAPIFYTVPLQLLSYHVALIKGTDVDQP
RNLAKSVTVE