Protein Info for HER17_RS21605 in Pectobacterium carotovorum WPP14

Annotation: 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 206 PF02527: GidB" amino acids 20 to 201 (182 residues), 209.6 bits, see alignment E=2.6e-66 TIGR00138: 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG" amino acids 25 to 204 (180 residues), 188.8 bits, see alignment E=3.7e-60 PF13847: Methyltransf_31" amino acids 65 to 142 (78 residues), 28.3 bits, see alignment E=1.4e-10

Best Hits

Swiss-Prot: 96% identical to RSMG_PECCP: Ribosomal RNA small subunit methyltransferase G (rsmG) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K03501, ribosomal RNA small subunit methyltransferase G [EC: 2.1.1.170] (inferred from 97% identity to eca:ECA4520)

MetaCyc: 75% identical to 16S rRNA m7G527 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11578 [EC: 2.1.1.170]

Predicted SEED Role

"rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.170

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (206 amino acids)

>HER17_RS21605 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (Pectobacterium carotovorum WPP14)
MRNTLDNLLNAAGIVISDKQKNLLIQYVDMLNKWNKAYNLTSVRDPQQMLVRHIMDSIVV
EPHLDGQRFIDVGTGPGLPGIPLAIVRPESHFTLLDSLGKRVRFLRQVQHELQLENITPV
QSRVEEFPAEPPFDGVISRAFASLQDMISWCSHLPARPAGRFYALKGVLPEDELSSLPQG
VLLDRVVRLSVPDLEGERHLIVLKPN