Protein Info for HER17_RS21470 in Pectobacterium carotovorum WPP14

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 929 PF02739: 5_3_exonuc_N" amino acids 9 to 170 (162 residues), 200.7 bits, see alignment E=2.6e-63 TIGR00593: DNA polymerase I" amino acids 9 to 929 (921 residues), 951.7 bits, see alignment E=2.5e-290 PF01367: 5_3_exonuc" amino acids 171 to 271 (101 residues), 109.2 bits, see alignment E=2.4e-35 PF01612: DNA_pol_A_exo1" amino acids 331 to 517 (187 residues), 145.3 bits, see alignment E=3.3e-46 PF00476: DNA_pol_A" amino acids 552 to 927 (376 residues), 548.8 bits, see alignment E=1.3e-168

Best Hits

Swiss-Prot: 76% identical to DPO1_ECOLI: DNA polymerase I (polA) from Escherichia coli (strain K12)

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 77% identity to cko:CKO_03151)

MetaCyc: 76% identical to DNA polymerase I (Escherichia coli K-12 substr. MG1655)
3.1.11.-; 3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (929 amino acids)

>HER17_RS21470 DNA polymerase I (Pectobacterium carotovorum WPP14)
MAQIAENPLILVDGSSYLYRAYHAFPPLTNSAGEATGAMYGVLNMLRSLLLQYQPSHVAV
VFDAKGKTFRDELFENYKAHRPPMPEDLREQIEPLHKMVKAMGLPLLAVSGVEADDVIGT
LAVQAEKAGKSVLISTGDKDMAQLVTPSVTLINTMNNSILGPQEVCDKYGIPPELIIDFL
ALMGDASDNIPGVPGVGEKTAQALLQGLGGLDSLYANLDKIAGLSFRGAKTMAPKLEQHK
EVAYLSYQLATIKTDVELELSCDQLTVNELDVDELHRLFSRYEFKRWLSDVESGTWMQGK
KSSQPVQAVSSAVVEQVVEEDNAPTLSTDGYVTILDEKTLLDWIERLKQAEVFAFDTETD
GLDTLTANLIGLSFAIKPGEAAYLPLAHDYLDAPEQLDRTKVLALFKPLLEDEKLLKIGQ
NLKFDKGVMQRYDIDLRGIAFDTMLESYVLDSVAGRHDMDSLAERYLSHKTITFEEIAGK
GKNQLTFNQIALEQAGPYAAEDADVTLHLHQKLWGKLQPHTDLCQVFQTIDMPLVPVLSR
IERTGVLIDPAILAEHSKELTIRLAELETQAYELAGEEFNLSSTKQLQGILYEKQKLPIL
KKTPKGAPSTNEEVLAELALGYPLPKLILEYRGLAKLKSTYTDKLPLMINPATKRVHTSY
HQAVTATGRLSSSDPNLQNIPVRNDEGRRIRQAFIAPQGYSIVAADYSQIELRIMAHLSG
DKGLLNAFANGLDIHRATASEVFGTALDKVTSEQRRSAKAINFGLIYGMSAFGLSRQLNI
PRSESQKYMNLYFERYPGVQDYMERTRQQAAEHGYVSTLDGRRLYLPDIHSRNAMARKGA
ERAAINAPMQGTAADIIKKAMIAIDDWLRKDTPKVKMIMQVHDELVFEIHDSVIEESISK
IKVLMEGCMQLNVPLQVDIGTGMNWDEAH