Protein Info for HER17_RS20390 in Pectobacterium carotovorum WPP14

Annotation: HesA/MoeB/ThiF family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00899: ThiF
  • amino acids 20 to 254 (235 residues), 223.8 bits, E=7.9e-71

Best Hits

Swiss-Prot: 61% identical to THIF_ECOLI: Sulfur carrier protein ThiS adenylyltransferase (thiF) from Escherichia coli (strain K12)

KEGG orthology group: K03148, adenylyltransferase [EC: 2.7.7.-] (inferred from 92% identity to pct:PC1_0214)

MetaCyc: 61% identical to sulfur carrier protein ThiS adenylyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-9789 [EC: 2.7.7.73]

Predicted SEED Role

"Sulfur carrier protein adenylyltransferase ThiF" in subsystem Thiamin biosynthesis

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of siderophore group nonribosomal peptides
• Lipopolysaccharide biosynthesis
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.- or 2.7.7.73

Protein Sequence (264 amino acids)

>HER17_RS20390 HesA/MoeB/ThiF family protein (Pectobacterium carotovorum WPP14)
MVTTHHPAPAGLSDSEFMRYSRQLMLEDIGPEGQEKLKVAHVLLVGLGGLGAPASLYLAA
AGVGTLLLADDDSLHISNLQRQILYRTSDTDKPKAVLAQRQLQALNPHSEAIAITERLSG
DALYSAVSRVDLVLDCSDNMTTRHAVNAACFSAGKPLISGSAVGFSGQLAVFTPPYHAGC
YACLYPDTTEPQRNCRTAGVLGPVVGVIGTLQALEAIKLLAGIPSALDGKLRMFNGKQQS
WNTLQLTRAPHCPVCGGKHENHAE