Protein Info for HER17_RS20370 in Pectobacterium carotovorum WPP14

Annotation: NAD(+) diphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF09297: zf-NADH-PPase
  • amino acids 93 to 124 (32 residues), 38.3 bits, 8.5e-14
• PF00293: NUDIX
  • amino acids 131 to 240 (110 residues), 67.6 bits, E=1.2e-22

Best Hits

Swiss-Prot: 97% identical to NUDC_PECAS: NADH pyrophosphatase (nudC) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K03426, NAD+ diphosphatase [EC: 3.6.1.22] (inferred from 97% identity to eca:ECA0234)

MetaCyc: 72% identical to RNA decapping hydrolase (Escherichia coli K-12 substr. MG1655)
NAD(+) diphosphatase. [EC: 3.6.1.22]; RXN0-7346 [EC: 3.6.1.22]

Predicted SEED Role

"NADH pyrophosphatase (EC 3.6.1.22)" in subsystem Nudix proteins (nucleoside triphosphate hydrolases) (EC 3.6.1.22)

MetaCyc Pathways

• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (2/3 steps found)
• NAD salvage (plants) (5/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.22

Protein Sequence (260 amino acids)

>HER17_RS20370 NAD(+) diphosphatase (Pectobacterium carotovorum WPP14)
MEQTLKGDETGWWVVSDAVQIWLPQGELPRGTAIEWSLQGKTARQIGEWQGLPVWLVCQG
RDSDMASVRQLLDQDVGLFQLAGRGVQLAEFYRSHRFCGYCGHEMVRSKTELACLCHHCK
ERYYPQIAPCIIVAIRRGDEILLAQHNRHRGNMYTVLAGFVEVGETLEQTVVREVMEESQ
IQIKNLRYVSSQPWPFPHSLMMAFMADYAGGDIKHDPKELRDAGWFRYDQLPQLPPPGTV
ARRLIEDTVVLCRTYHENEG