Protein Info for HER17_RS19985 in Pectobacterium carotovorum WPP14

Annotation: serine endoprotease DegQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR02037: peptidase Do" amino acids 40 to 454 (415 residues), 536 bits, see alignment E=3.6e-165 PF13365: Trypsin_2" amino acids 93 to 229 (137 residues), 118.9 bits, see alignment E=8e-38 PF00089: Trypsin" amino acids 94 to 254 (161 residues), 73.3 bits, see alignment E=6.7e-24 PF00595: PDZ" amino acids 264 to 345 (82 residues), 50 bits, see alignment E=8.2e-17 amino acids 374 to 444 (71 residues), 46.6 bits, see alignment E=9.3e-16 PF13180: PDZ_2" amino acids 286 to 357 (72 residues), 56.2 bits, see alignment E=9.1e-19 amino acids 386 to 436 (51 residues), 31.3 bits, see alignment 5.1e-11 PF17820: PDZ_6" amino acids 294 to 342 (49 residues), 35.6 bits, see alignment 1.7e-12 amino acids 395 to 445 (51 residues), 38.9 bits, see alignment 1.5e-13

Best Hits

Swiss-Prot: 70% identical to DEGQ_ECOLI: Periplasmic pH-dependent serine endoprotease DegQ (degQ) from Escherichia coli (strain K12)

KEGG orthology group: K04772, serine protease DegQ [EC: 3.4.21.-] (inferred from 99% identity to pct:PC1_0289)

MetaCyc: 70% identical to periplasmic serine endoprotease (Escherichia coli K-12 substr. MG1655)
3.4.21.-

Predicted SEED Role

"Outer membrane stress sensor protease DegQ, serine protease" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>HER17_RS19985 serine endoprotease DegQ (Pectobacterium carotovorum WPP14)
MKKTSLLFSALAMSIGLTLSTLPAANAALPAVVQGQQTPSLAPMLEKVLPAVVSVHVEGT
QVQRQRVPEEFKFFFGPNFPTDKQNSRPFEGLGSGVIIDAAKGYVLTNNHVINNADKIRV
QLNDGREYEAKLIGRDEQTDIALLQLNDAKNLVSVKMADSDQLRVGDFAVAVGNPFGLGQ
TATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGALVNLNGELIGINTAILAPGGGN
IGIGFAIPSNMAQNLAQQLVEFGEVKRGLLGIKGSEMTSEMAKAFNVDAQRGAFVSEVLP
KSAASKAGIKAGDVLTTLDGKPISSFAELRAKVGTTAPGKTVKIGLLRDGKPQEVSVVLD
NSSSASTSAETLSPSLQGASLTNGQLKDGSKGVQIDNVAKDTPAAQVGLQKGDIIIGVNR
ERIENITQLRKLLEAKPSVLALNIVRGEETIYLLLR