Protein Info for HER17_RS19650 in Pectobacterium carotovorum WPP14

Annotation: sucrose-specific PTS transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 109 to 133 (25 residues), see Phobius details amino acids 144 to 168 (25 residues), see Phobius details amino acids 211 to 233 (23 residues), see Phobius details amino acids 245 to 267 (23 residues), see Phobius details amino acids 279 to 308 (30 residues), see Phobius details amino acids 328 to 350 (23 residues), see Phobius details amino acids 360 to 381 (22 residues), see Phobius details amino acids 388 to 408 (21 residues), see Phobius details amino acids 428 to 449 (22 residues), see Phobius details TIGR01996: PTS system, sucrose-specific IIBC component" amino acids 4 to 448 (445 residues), 636.3 bits, see alignment E=4.1e-195 PF00367: PTS_EIIB" amino acids 10 to 41 (32 residues), 51.2 bits, see alignment (E = 6.8e-18) TIGR00826: PTS system, glucose-like IIB component" amino acids 28 to 111 (84 residues), 78.3 bits, see alignment E=6.6e-26 PF02378: PTS_EIIC" amino acids 110 to 395 (286 residues), 186.3 bits, see alignment E=7.9e-59 TIGR00852: PTS system, maltose and glucose-specific subfamily, IIC component" amino acids 153 to 437 (285 residues), 299.9 bits, see alignment E=2.8e-93

Best Hits

Swiss-Prot: 82% identical to PTSBC_KLEPN: PTS system sucrose-specific EIIBC component (scrA) from Klebsiella pneumoniae

KEGG orthology group: K02809, PTS system, sucrose-specific IIB component [EC: 2.7.1.69] K02810, PTS system, sucrose-specific IIC component (inferred from 98% identity to eca:ECA0362)

MetaCyc: 82% identical to Enzyme IIscr (Klebsiella pneumoniae)
SUCROSEPHOSPHO-RXN [EC: 2.7.1.211]

Predicted SEED Role

"PTS system, sucrose-specific IIB component (EC 2.7.1.69) / PTS system, sucrose-specific IIC component (EC 2.7.1.69)" in subsystem Sucrose utilization (EC 2.7.1.69)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.211 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>HER17_RS19650 sucrose-specific PTS transporter subunit IIBC (Pectobacterium carotovorum WPP14)
MDINATAAALIPLLGGKENIASAAHCATRLRLVLNDDSLADKKAIENVDGVKGCFQNAGQ
MQIIFGTGLVNKVYAEFIKAAGISESSKSEAASIAAKKLNPLQRLARLLSNIFVPIMPAI
IASGLLMGLLGMIKTYGWVDSSSAIFVMLDMFSSAAFIILPVLIGFTAAREFGGNPYLGA
TLGGILTHPALTNAWGVAGGFQTMHFFGMDIAMIGYQGTVFPVLLAVWFMSIVEKRLRKI
VPDALDIIVTPFLTVIISGFVAMLLIGPAGRALGDGISLVLSTLIAHAGWLAGLLFGGLY
SVIVITGVHHSFHAIEAGLLGNPNIGVNFLLPIWAMANVAQGGACLAVYFKTRDAKTRAI
AVPAGLSCLLGITEAAIFGINLRFIKPFLAALAGGALGGAWVVFNHVNMTAVGLTGFPGL
AIVQGGSMLNYLIGMLIAFGAAFILSLLLKYKTDSE