Protein Info for HER17_RS19460 in Pectobacterium carotovorum WPP14

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 956 transmembrane" amino acids 503 to 524 (22 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 936 (936 residues), 1266.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 631 (618 residues), 543.8 bits, see alignment E=6.2e-167 PF09334: tRNA-synt_1g" amino acids 42 to 188 (147 residues), 30.2 bits, see alignment E=5.2e-11 PF13603: tRNA-synt_1_2" amino acids 247 to 341 (95 residues), 29.1 bits, see alignment E=1.7e-10 PF08264: Anticodon_1" amino acids 674 to 830 (157 residues), 151.8 bits, see alignment E=3.1e-48 PF10458: Val_tRNA-synt_C" amino acids 891 to 949 (59 residues), 60.2 bits, see alignment 4.9e-20

Best Hits

Swiss-Prot: 87% identical to SYV_ECOLI: Valine--tRNA ligase (valS) from Escherichia coli (strain K12)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 75% identity to avr:B565_0259)

MetaCyc: 87% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (956 amino acids)

>HER17_RS19460 valine--tRNA ligase (Pectobacterium carotovorum WPP14)
METKYNPQDIEQPLYEHWEKQGYFKPHGDTSKESFSIMIPPPNVTGSLHMGHAFQQTIMD
TLIRYQRMQGKNTLWQAGTDHAGIATQMVVERKIAAEEGKTRHDYGREAFIDKIWQWKGE
SGGNITNQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRLYKEDLIYRGKRLVNWDPKLR
TAISDLEVENREVKGSMWHLRYPLADGVKTADGKDYLVVATTRPETVLGDTGVAVNPEDP
RYKDLIGKEVILPLIGRRIPIVGDEHADMEKGTGCVKITPAHDFNDYEVGKRHQLPMVNI
LTFDGDIRQSAEIFDTNGEASTAYNSDIPEAFQGLERFAARKALVAAFDELGLLEEIKAH
DLTVPYGDRGGVVIEPMLTDQWYVRAAVLAKPAVEAVEDGRIQFVPKQYENMYFSWMRDI
QDWCISRQLWWGHRIPAWYDANGNVYVGRTEAEVRSENNLADDVVLNQDEDVLDTWFSSG
LWTFSTLGWPEQTPDLKAFHPSSVMVSGFDIIFFWIARMIMLTMHFIKDEDGKSQVPFHT
VYMTGLIRDEEGQKMSKSKGNVIDPLDMVDGISLEALLEKRTGNMMQPQLAEKIRKRTEK
QFPNGIEPHGTDALRFTLAALASTGRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEDQD
CGFGAGEKVLSLADRWILAEFNRTVKAYREALDSYRFDIAAGILYEFTWNQFCDWYLELT
KPVMNGGSEAELRGTRHTLVTVLEALLRLAHPIIPFITETIWLRVKTLKGISAAVRTNES
IMLQPFPEFDAAQEDTLALNDLEWIKQAIIAVRNIRAEMNIAPGKPLEVLLRDVTAEAQR
RVEENRSFIQTLARLESITLLPAGDKGPVSVTKLIDGAELLIPMAGLIDKAAELDRLTKE
VAKIEAEIERIESKLSNEGFVARAPEAVVAKEREKLDGYAVAKAKLLEQHAVIAAL