Protein Info for HER17_RS19085 in Pectobacterium carotovorum WPP14

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 TIGR00416: DNA repair protein RadA" amino acids 1 to 454 (454 residues), 773.3 bits, see alignment E=3.9e-237 PF18073: Rubredoxin_2" amino acids 9 to 36 (28 residues), 43.3 bits, see alignment (E = 9.3e-15) PF06745: ATPase" amino acids 78 to 152 (75 residues), 39.8 bits, see alignment E=1.4e-13 PF13481: AAA_25" amino acids 88 to 225 (138 residues), 48.4 bits, see alignment E=3.6e-16 PF09848: SLFN-g3_helicase" amino acids 97 to 184 (88 residues), 21.6 bits, see alignment E=4.8e-08 PF13541: ChlI" amino acids 348 to 433 (86 residues), 37.6 bits, see alignment E=7.4e-13 PF05362: Lon_C" amino acids 355 to 454 (100 residues), 31.8 bits, see alignment E=4.3e-11

Best Hits

Swiss-Prot: 92% identical to RADA_SALTY: DNA repair protein RadA (radA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 99% identity to pct:PC1_0449)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (460 amino acids)

>HER17_RS19085 DNA repair protein RadA (Pectobacterium carotovorum WPP14)
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASVSRSDRLTGYAGESAGV
SRVQKLSEISLEALPRFSTGFQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLS
ENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIEQICLIAEQEQPKLMVIDSIQ
VMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSV
MVVWEGTRPLLVEIQALVDQSMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFV
NVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEVGLAGEIRPVPSGQERITEAA
KHGFKRAIVPHANMPKKVPASMQVFGVKKLADALAILDDL