Protein Info for HER17_RS18680 in Pectobacterium carotovorum WPP14

Annotation: 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05175: MTS
  • amino acids 200 to 371 (172 residues), 180.9 bits, E=1.6e-57
• PF06325: PrmA
  • amino acids 230 to 306 (77 residues), 29.3 bits, E=6.3e-11

Best Hits

Swiss-Prot: 96% identical to RLMG_PECAS: Ribosomal RNA large subunit methyltransferase G (rlmG) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00564, ribosomal RNA small subunit methyltransferase C [EC: 2.1.1.172] (inferred from 96% identity to pct:PC1_0536)

MetaCyc: 61% identical to 23S rRNA m2G1835 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11635 [EC: 2.1.1.174]

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmG (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-, 2.1.1.172

Use Curated BLAST to search for 2.1.1.- or 2.1.1.172 or 2.1.1.174

Protein Sequence (379 amino acids)

>HER17_RS18680 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG (Pectobacterium carotovorum WPP14)
MSQLELETCNLTLVRYPQVAENSALQAWDAADEYLLRELANMEIAPGPRLIFNDTFGALA
CGLQAQSPVSISDSYLSQLATRHNLELNGYAVDAVTLLDSMAELPAAPALVVIKIPKTIA
LLEHQLRMLRKVVTPQTRIIAGAKARDIHTSTLQLFERVMGPTKTSLAWKKARLVHCEWA
ELKVSEQSLTTEWELDGYGYRIQNHANVYSRNGLDIGARFFMQHLPEQIDGKIIDLGCGN
GVIGLAALEANPEATVGFFDESYMAVASSQINVEVNRPQDIERCSFVVNHGLARVKRDTL
QAVLCNPPFHQQQAVTDEIAWQMFMDARRCLQVGGELRIVGNRHLDYFHKLKRLFGNCET
LASNTKFAVLRAVKTGSSR