Protein Info for HER17_RS18130 in Pectobacterium carotovorum WPP14

Annotation: thymidine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02643: thymidine phosphorylase
  • amino acids 2 to 438 (437 residues), 747.4 bits, E=5.3e-229
• TIGR02644: pyrimidine-nucleoside phosphorylase
  • amino acids 5 to 423 (419 residues), 546.5 bits, E=3.9e-168
• PF02885: Glycos_trans_3N
  • amino acids 5 to 67 (63 residues), 72.1 bits, E=3.8e-24
• PF00591: Glycos_transf_3
  • amino acids 79 to 309 (231 residues), 187.7 bits, E=4.6e-59
• PF07831: PYNP_C
  • amino acids 351 to 423 (73 residues), 81.6 bits, E=4e-27

Best Hits

Swiss-Prot: 98% identical to TYPH_PECAS: Thymidine phosphorylase (deoA) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 99% identity to pct:PC1_0600)

MetaCyc: 81% identical to thymidine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]; Thymidine phosphorylase. [EC: 2.4.2.2, 2.4.2.3, 2.4.2.4]

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleosides degradation (6/6 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• pyrimidine ribonucleosides degradation (2/2 steps found)
• superpathway of pyrimidine ribonucleosides degradation (2/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.3

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3 or 2.4.2.4

Protein Sequence (442 amino acids)

>HER17_RS18130 thymidine phosphorylase (Pectobacterium carotovorum WPP14)
MFLIQEIIRKKRDGKALSEEEIRFFINGIRDNTVSEGQIAALAMTIYFHDMSMDERVALT
LAMRDSGTVLDWKSLNLNGPLVDKHSTGGVGDVTSLMLGPMVAACGGYVPMISGRGLGHT
GGTLDKLEAIPGLDIFPNDEDFRRIIQQVGVAIIGQTSSLAPADKRFYATRDITATVDSI
PLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSEQLAQAIVGVANNAGCRTSALLT
DMNQVLASSAGNALEVREAVRFLTGESRNPRLYDVTMALCGEMLLAGGLASSADDAHSRL
QAVLDNGKAAEVFGRMVAAQRGPGDFVEHYDRYLPVATLSKPVFATREGIVTGMDTRALG
MAVVSLGGGRRQATDTIDYSVGLDSMISLGERVDAQRPLAVIHANTEAQWQLAANEVRAA
IQLGDTAPEKPPMVYRRVSAEA