Protein Info for HER17_RS18005 in Pectobacterium carotovorum WPP14

Annotation: glycine cleavage system aminomethyltransferase GcvT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 TIGR00528: glycine cleavage system T protein" amino acids 2 to 363 (362 residues), 487.1 bits, see alignment E=1.5e-150 PF01571: GCV_T" amino acids 7 to 259 (253 residues), 281.1 bits, see alignment E=7.4e-88 PF08669: GCV_T_C" amino acids 285 to 362 (78 residues), 43.4 bits, see alignment E=2.7e-15

Best Hits

Swiss-Prot: 97% identical to GCST_PECCP: Aminomethyltransferase (gcvT) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00605, aminomethyltransferase [EC: 2.1.2.10] (inferred from 97% identity to pct:PC1_0622)

Predicted SEED Role

"Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 2.1.2.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 2.1.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (371 amino acids)

>HER17_RS18005 glycine cleavage system aminomethyltransferase GcvT (Pectobacterium carotovorum WPP14)
MAKQTPLYQQHLADGAKMVDFHGWMMPLHYGSQLDEHHIVRREAGIFDVSHMTIVDLHGV
RTREFLRYLLANDVAKLTQPGKALYTGMLNASGGVIDDLIVYFLTEDNFRLVVNSATREK
DLAWIGQHAAPFGVEIRERDDLALVAVQGPQAQEKVQEILKAKGLSDTDVAAVASMKPFF
GKQAGDFFVATTGYTGEAGYEIALPNEQVVDFWQQLLAVGVKPCGLGARDTLRLEAGMNL
YGQDMDEGISPLAANMGWTIAWQPEDREFIGREALTHQREKGTDQLVGLVLTEKGVLRND
LPVRFTDSDGVVREGVITSGSFSPTLGVSIALARVPVGIGEQAIVQIRNRELPVHVAKPG
FVRAGKAIVQY