Protein Info for HER17_RS17995 in Pectobacterium carotovorum WPP14

Annotation: aminomethyl-transferring glycine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02347: GDC-P
  • amino acids 16 to 440 (425 residues), 606.6 bits, E=3.8e-186
  • amino acids 462 to 739 (278 residues), 35.7 bits, E=7.3e-13
• TIGR00461: glycine dehydrogenase
  • amino acids 17 to 950 (934 residues), 1662.3 bits, E=0
• PF21478: GcvP2_C
  • amino acids 781 to 902 (122 residues), 200.4 bits, E=1.1e-63

Best Hits

Swiss-Prot: 66% identical to GCSP2_PSEF5: Glycine dehydrogenase (decarboxylating) 2 (gcvP2) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 68% identity to avr:B565_1479)

MetaCyc: 82% identical to glycine decarboxylase (Escherichia coli K-12 substr. MG1655)
Glycine dehydrogenase (decarboxylating). [EC: 1.4.1.27, 1.4.4.2]

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• glycine degradation (3/3 steps found)
• photorespiration II (7/10 steps found)
• folate transformations I (9/13 steps found)
• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.27 or 1.4.4.2

Protein Sequence (957 amino acids)

>HER17_RS17995 aminomethyl-transferring glycine dehydrogenase (Pectobacterium carotovorum WPP14)
MTQTLSQLEHDGAFIERHIGPSVSQQQHMLSVVGATSLDALIRQIVPVDIQLPSPPAVGE
AVTEHEALAELKAIAGRNQRYKSYIGMGYSAVLMPPVILRNLLENPGWYTAYTPYQPEVS
QGRLEALLNFQQVTQDLTGLDLASASLLDEATAAAEAMAMAKRISKLKQAERFFVADDVH
PQTLDVVRTRAETFGFDIVVGKAEEALKDDAVFGVLLQQAGTTGELHDYSNLMAALKARK
VVTCVASDIMALVLLTAPGKQGADIVFGSAQRFGVPMGYGGPHAAFFACRDEHKRAMPGR
IIGVSRDAAGNTALRMAMQTREQHIRREKANSNICTSQVLLANIAGMYAVFHGPEGLKRI
AGRIHRLTDILAAGLTQGGLLLRHRSWFDTLTIEVADKDAVLSRALSFGINLRSDLASAV
GITLDEATTREDVLALFAVLLGDDHGLDIDALDAAIGQKAATIPAGLVRHDAILSHPVFN
RYHSETEMMRYLHRLARKDLALNQAMIPLGSCTMKLNAAAEMLPITWPEFAELHPFCPPE
QALGYRQMIEQLSGWLVQLTGYDAICMQPNSGAQGEYAGLLAIRRYHESRNEAGRNLCLI
PSSAHGTNPASAQMAGMDVVVVACDKQGNIDLHDLREKAQAAGEQLSCIMVTYPSTHGVY
EETIREVCQIVHQYGGQVYLDGANMNAQVGITTPGYIGADVSHLNLHKTFCIPHGGGGPG
MGPIGVKAHLAPFVPGHQVVKIEGVLTEQGAVSAAPFGSASILPISWMYIRMMGAEGLKQ
ASQMAILNANYIATRLQQAYPVLYTGRDGRVAHECILDIRPLKESTGISEMDIAKRLIDY
GFHAPTMSFPVAGTLMVEPTESESQVELDRFIDAMLAIRTEINRVAQGEWPLDDNPLVNA
PHTQAELVADWAHPYSRELAVFPAGSEHKYWPSVKRLDDVYGDRNLFCSCVPMSEYA