Protein Info for HER17_RS17955 in Pectobacterium carotovorum WPP14

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 420 transmembrane" amino acids 14 to 39 (26 residues), see Phobius details amino acids 51 to 70 (20 residues), see Phobius details amino acids 81 to 101 (21 residues), see Phobius details amino acids 107 to 130 (24 residues), see Phobius details amino acids 150 to 167 (18 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 227 to 244 (18 residues), see Phobius details amino acids 264 to 288 (25 residues), see Phobius details amino acids 296 to 317 (22 residues), see Phobius details amino acids 323 to 342 (20 residues), see Phobius details amino acids 354 to 374 (21 residues), see Phobius details amino acids 386 to 406 (21 residues), see Phobius details PF01306: LacY_symp" amino acids 7 to 415 (409 residues), 638.2 bits, see alignment E=8.2e-196 TIGR00882: oligosaccharide:H+ symporter" amino acids 11 to 406 (396 residues), 634 bits, see alignment E=4.6e-195 PF12832: MFS_1_like" amino acids 15 to 354 (340 residues), 82.4 bits, see alignment E=5.3e-27 PF07690: MFS_1" amino acids 21 to 319 (299 residues), 69 bits, see alignment E=5.3e-23

Best Hits

Swiss-Prot: 67% identical to MELY_ENTCC: Melibiose permease (melY) from Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)

KEGG orthology group: K02532, MFS transporter, OHS family, lactose permease (inferred from 98% identity to pct:PC1_0632)

MetaCyc: 59% identical to lactose permease (Escherichia coli K-12 substr. MG1655)
RXN-17755; RXN0-7215; RXN0-7217; TRANS-RXN-24; TRANS-RXN-94

Predicted SEED Role

"Lactose permease" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Lactose and Galactose Uptake and Utilization or Lactose utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (420 amino acids)

>HER17_RS17955 MFS transporter (Pectobacterium carotovorum WPP14)
MPTTSCYYKNNRNFWIFGAFFFLYFFIMATCFPFLPIWLADVIGLNKTDTGIVFSFLSLF
AILFQPFLGILSDKLGMKKHLLWVISILLLFFAPFFLYVFAPLLKINVILGALVGGLYIG
FSFSAGAGAIEAYIERISRQHHFEYGKARMFGCFGWGICASTAGMLFNINPDIVFWMGSG
SAIILIVLLCLAKTESNPTAAVMDSLGANASPFSVKLALGLLANRQFWLLVLYVVGVACI
YDVYDQQFANFFKSFFSSQEQGNQIFGFVTTAGEAANALVMFCTPWIINRIGAKNALLVA
GTIMSIRILGSACATSVTEVIILKMLHAFEVPLLLIGIFKYIANTFDSRLSATIYLVGFQ
FAKQFMAIFLSSAAGNLYDRIGFNQTYLILGGIALTFTAISAFTLSSSRSSAGMRSQAAH