Protein Info for HER17_RS17930 in Pectobacterium carotovorum WPP14

Annotation: tRNA-modifying protein YgfZ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 TIGR03317: folate-binding protein YgfZ" amino acids 194 to 258 (65 residues), 87.9 bits, see alignment E=1.6e-29 PF21130: YgfZ_barrel" amino acids 250 to 317 (68 residues), 121 bits, see alignment E=8.8e-40

Best Hits

Swiss-Prot: 95% identical to YGFZ_PECAS: tRNA-modifying protein YgfZ (ECA0758) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K06980, (no description) (inferred from 95% identity to pct:PC1_0637)

Predicted SEED Role

"Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (333 amino acids)

>HER17_RS17930 tRNA-modifying protein YgfZ (Pectobacterium carotovorum WPP14)
MVNQHTAHQRPFASQPPFASAQLAATLISLDDWALVTMVGPDTVKYLQGQVTADVGALPD
DGHTLCAHCDAKGKMWSNLRLFHHGEGFAFIERRNLRDAQLSELKKYAVFSKTTIAPDDN
AILLGAAGAGIRELLASVFSQLPDVEHPVVQHEGATLLHFAHPAERFLLVLSPEHSASLL
EQLGDKVSLNDSCQWLTLDIEAGQPIIDSANSAQFIPQATNLQALNGISFSKGCYTGQEM
VARAKYRGANKRALYWLAGKANKVPQAGDDLELQLGENWRRTGTVLAASQLQNGDVWVQA
VLNNDLTAENVLRVREDAESQLTVQPLPYEITD