Protein Info for HER17_RS17225 in Pectobacterium carotovorum WPP14

Annotation: sulfate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 28 to 336 (309 residues), 531.1 bits, see alignment E=4.5e-164 PF01547: SBP_bac_1" amino acids 46 to 282 (237 residues), 54.4 bits, see alignment E=4e-18 PF13531: SBP_bac_11" amino acids 53 to 290 (238 residues), 122.8 bits, see alignment E=4e-39 PF13416: SBP_bac_8" amino acids 121 to 282 (162 residues), 31.2 bits, see alignment E=3.9e-11 PF13343: SBP_bac_6" amino acids 139 to 301 (163 residues), 32.8 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 79% identical to CYSP_ECOLI: Thiosulfate-binding protein (cysP) from Escherichia coli (strain K12)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 97% identity to pwa:Pecwa_1002)

MetaCyc: 79% identical to thiosulfate/sulfate ABC transporter periplasmic binding protein CysP (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (342 amino acids)

>HER17_RS17225 sulfate ABC transporter substrate-binding protein (Pectobacterium carotovorum WPP14)
MNQVQVKGWLVKGSLALSLLLGGQSAVSATELLNSSYDVSRELFVALNPGFEKQWLSQHP
NDPLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDRGQLIPADWQTRLPNNSS
PFYSTMAFLVRKGNPKGIHDWNDLVRDDVKLIFPNPKTSGNARYTYLAAWGATSKANGGD
EAKTREWVKRFLANVEVFDTGGRGATTTFVERQLGDVLISFESEVNNIRNQYGAENYEVI
VPKVNVLAEFPVAWIDKNVEKNGTEQAAKAYLNYLYSPEAQKVITDFYYRVNNPELTQTL
KSRFPETTLFRVEDQFGAWPQVMKTHFDTGGELDKLLAAGRQ