Protein Info for HER17_RS16525 in Pectobacterium carotovorum WPP14

Annotation: exodeoxyribonuclease V subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1193 TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 9 to 1179 (1171 residues), 1315.9 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 16 to 442 (427 residues), 171.8 bits, see alignment E=5e-54 PF13245: AAA_19" amino acids 21 to 431 (411 residues), 49.3 bits, see alignment E=1.2e-16 PF13361: UvrD_C" amino acids 690 to 816 (127 residues), 42.3 bits, see alignment E=1.3e-14

Best Hits

Swiss-Prot: 63% identical to RECB_ECOLI: RecBCD enzyme subunit RecB (recB) from Escherichia coli (strain K12)

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 93% identity to eca:ECA0995)

MetaCyc: 63% identical to exodeoxyribonuclease V subunit RecB (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]; Exodeoxyribonuclease V. [EC: 5.6.2.4, 3.1.11.5]; RXN-19004 [EC: 5.6.2.4, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1193 amino acids)

>HER17_RS16525 exodeoxyribonuclease V subunit beta (Pectobacterium carotovorum WPP14)
MKNAAPQSLDVMTLPLLGERLIEASAGTGKTYTLAALYLRLLLGLGKQAAYPRPLLVEEI
LVVTFTEAATEELRERIRARIHALRIACLRKSAQGEEAEKPKDTSLGQLLAEISDHRDAA
DVLLAAERQMDEAAIYTIHGFCQRMLSTNAFESGVLFEQVLIEDEQPLRRQACADFWRRY
CYPLPVEVARIVGLEWKGPENLLADLAPYLHGEAPVFRLPPQEDETLVSRHENIVATIDA
YKQRWLASAAELEALISASGVDKRSYSSKHLPNWLQKVTLWAEQPTLDYQLPKDLVRFAQ
QTLIEKTKKGEPPVHSLFEETERLLEAPLSLRDLVIVRALSAIRDSVREEKRQRAELGFD
DLLSRLDVALQQPGGEQLASAIRERYPVAMIDEFQDTDPQQYRIFRTLYVGQSQCGLLLI
GDPKQAIYAFRGADIFTYMHARGEVAAHYTLGTNWRSSHQMVCGVNRLFERLENPFIFQN
IPFLPVKPAESKRGLVFEVAGQPQPALQFWLTGSEPIGVGDYQQRMARQCAAQIRDWLAA
SQRNEAWLITDDSRRLVRASDMSVLVRSRREASLIRDALSRLSIPSVYLSNRDSVFSTPE
ASDMLWLLQAVLAPEQERTLRSAMATALMGLDAGQVDALGQSEAAWDALVDEFAGYRALW
RQRGVLPMLRALMSQHQLAENLLASAEGERRITDILHIGELLQDASATLDSEHALVRWLS
QQIVQPNPQAENQQLRLESDRHLVQIVTIHKSKGLEYPLVWLPFISNFRVQDQGVYHDRE
SYQAVLDLQNNEESQTLAKEERLAEDLRLLYVALTRSIYHCSVGVAPVQRSRKKDGSSDM
HQSALGYLLQRGKEAEAATLVSELEGMVGDGVALTPLQATEEQRLQPDRPELAELRARHI
ERQLRDGWRVTSYSGLQQHGSASAQDLVPRLDIEAIGERQEADETQLTPHTFPRGASPGT
FLHSLFETLDFTQPVEDDWLAEQLQLQGFEAHWHPVLKTWMDTLLHTSLNEQGIMLSSLD
NADKQAELQFYIPIDAPIQAAQLDRLAKHHDPLSARCPALSFQQVKGMLKGFIDLVFRWE
GRYYLLDYKSNWLGPDASAYTQTAMMQSMAEHRYDLQYQLYTLALHRYLRHRIADYDYER
HFGGVFYLFLRGVEASHPGNGIFACRPPAEFVFGLDALFKGKTPDDADTGIPS