Protein Info for HER17_RS16305 in Pectobacterium carotovorum WPP14

Annotation: phosphatidate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 signal peptide" amino acids 5 to 6 (2 residues), see Phobius details transmembrane" amino acids 7 to 23 (17 residues), see Phobius details amino acids 28 to 46 (19 residues), see Phobius details amino acids 56 to 76 (21 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 118 to 138 (21 residues), see Phobius details amino acids 150 to 171 (22 residues), see Phobius details amino acids 192 to 211 (20 residues), see Phobius details amino acids 217 to 236 (20 residues), see Phobius details amino acids 265 to 282 (18 residues), see Phobius details PF01148: CTP_transf_1" amino acids 4 to 279 (276 residues), 244.5 bits, see alignment E=9.1e-77

Best Hits

Swiss-Prot: 76% identical to CDSA_ECO57: Phosphatidate cytidylyltransferase (cdsA) from Escherichia coli O157:H7

KEGG orthology group: K00981, phosphatidate cytidylyltransferase [EC: 2.7.7.41] (inferred from 99% identity to eca:ECA1037)

MetaCyc: 76% identical to CDP-diglyceride synthetase (Escherichia coli K-12 substr. MG1655)
Phosphatidate cytidylyltransferase. [EC: 2.7.7.41]; 2.7.7.41 [EC: 2.7.7.41]

Predicted SEED Role

"Phosphatidate cytidylyltransferase (EC 2.7.7.41)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.7.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (285 amino acids)

>HER17_RS16305 phosphatidate cytidylyltransferase (Pectobacterium carotovorum WPP14)
MLKYRLITAFILIPIVIAALFLLPPLGFTLVTLAVCMLAAWEWGQLAGFASYGQRLWLAI
LCGFLLALMLLSLPAYHYSVHIPQISIALWSSLVWWGVALLLVLFYPASASFWRHSRTLR
LVFGIMTIVPFFWGMVALRHYNYAINPFAGAWWLLYVMLLVWGADSGAYMFGKLFGKRKL
APKVSPGKTWEGFLGGLATSALISVLFSLYAPLTVAPSTLLICSIAAALASVLGDLTESM
FKREAGIKDSSHLIPGHGGVLDRIDSLTAAVPVFSCLMLLLFKVA