Protein Info for HER17_RS16140 in Pectobacterium carotovorum WPP14

Annotation: mannonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 TIGR00695: mannonate dehydratase" amino acids 1 to 393 (393 residues), 687.3 bits, see alignment E=3.4e-211 PF03786: UxuA" amino acids 1 to 388 (388 residues), 473.9 bits, see alignment E=2.6e-146

Best Hits

Swiss-Prot: 97% identical to UXUA_PECCP: Mannonate dehydratase (uxuA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01686, mannonate dehydratase [EC: 4.2.1.8] (inferred from 97% identity to pct:PC1_0987)

MetaCyc: 75% identical to D-mannonate dehydratase (Escherichia coli K-12 substr. MG1655)
Mannonate dehydratase. [EC: 4.2.1.8]

Predicted SEED Role

"Mannonate dehydratase (EC 4.2.1.8)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 4.2.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (398 amino acids)

>HER17_RS16140 mannonate dehydratase (Pectobacterium carotovorum WPP14)
MEQTWRWYGPNDPVSLDDVRQAGATGVVTALHHIPNGEIWSIEEIEKRKAELKAKGLVWS
VVESVPVHEEIKTQTGNYQQHIANYQQSLRNLAQCGIDTVCYNFMPVLDWTRTDLEYVLP
DGAKALRFDHIAFAAFELHILQRHGAEKDYTADEQAQAADYFRAMSEEDIARLTGNIIAG
LPGSEEGYTLEQFRGRLAEYDGIDHAKLREHLGHFLREIVPVAEAVGLRLAIHPDDPPRP
ILGLPRVMSTIDDMRWLKQTVDSLHNGFTFCTGSYGVRADNDLVTMLETFGDRVHFTHLR
ATCREDNPNSFHEGAHLQGDVDMVAIIKVILAEELRRQKAGDRRPIPMRPDHGHQMLDDL
KKKTNPGYSAIGRLKGLAELRGVELALKRMLFTELQQG