Protein Info for HER17_RS15625 in Pectobacterium carotovorum WPP14

Annotation: copper-exporting P-type ATPase CopA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 907 transmembrane" amino acids 259 to 279 (21 residues), see Phobius details amino acids 289 to 310 (22 residues), see Phobius details amino acids 322 to 344 (23 residues), see Phobius details amino acids 356 to 374 (19 residues), see Phobius details amino acids 508 to 530 (23 residues), see Phobius details amino acids 536 to 558 (23 residues), see Phobius details amino acids 850 to 869 (20 residues), see Phobius details amino acids 875 to 896 (22 residues), see Phobius details PF00403: HMA" amino acids 7 to 62 (56 residues), 43.9 bits, see alignment 6.2e-15 amino acids 73 to 128 (56 residues), 38.1 bits, see alignment 4.3e-13 amino acids 177 to 231 (55 residues), 55.8 bits, see alignment 1.2e-18 TIGR01511: copper-translocating P-type ATPase" amino acids 305 to 899 (595 residues), 624.3 bits, see alignment E=5.6e-191 TIGR01525: heavy metal translocating P-type ATPase" amino acids 324 to 899 (576 residues), 602.4 bits, see alignment E=2.6e-184 TIGR01494: HAD ATPase, P-type, family IC" amino acids 363 to 869 (507 residues), 301.2 bits, see alignment E=2.7e-93 PF00122: E1-E2_ATPase" amino acids 392 to 572 (181 residues), 174.9 bits, see alignment E=3.1e-55 PF00702: Hydrolase" amino acids 590 to 804 (215 residues), 138.8 bits, see alignment E=7.6e-44 PF08282: Hydrolase_3" amino acids 777 to 836 (60 residues), 30.1 bits, see alignment 1e-10

Best Hits

Swiss-Prot: 71% identical to COPA_ECO57: Copper-exporting P-type ATPase (copA) from Escherichia coli O157:H7

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 95% identity to eca:ECA1193)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (907 amino acids)

>HER17_RS15625 copper-exporting P-type ATPase CopA (Pectobacterium carotovorum WPP14)
MSQTIVLSLQGLTCGHCVQRVKKALEALPAVEQTNVTQQYARVSGDVDSQTLIDTIEQAG
YEAQLATTPDVSLQLSGLSCNHCVAATRKVLEAIPGVVATDVTTEQATVYGNVEATALIS
AIEEAGYHASVQENVHPKTEPLAQVATTPEALPAAESILPAMTTLATRDDDSVQLLLQGM
SCASCVNRVQTALQNVSGVTQARVNLAERSALVSGHAEPEALIAAVEQAGYGAEIIQDEE
ARRARQQQTSQQAIRRFQWQAALGLLLGVPLMLWGVLGGSMSLTPESQTPWLVIGILTLA
VMVFAGSHFYRNAWRSLRNGTATMDTLVALGTGAAWLYSITVNLWPDLFPIAARHLYYEA
SAMIIGLINLGHALEQRARQRSSRALERLLDLTPPTARVVTPEGEKSIPLSDVQSGMTLR
LTTGDRIPVDGEILQGEVWVDEAMLTGEPIPQAKTAGDTVHAGTLVSDGSVLFRADAIGN
QTTLSRIIRLVRQAQSSKPAIGQLADRISAVFVPVVVAIALLSGAVWFFVGPQPQIVYTL
IIVTTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDADALQQASKLDVLVFDKTGTL
TEGQPRVVAVHTFGDVTEVQALAWAASLEQGSNHPLAKAILERAENLALTPTEQFRTLRG
LGVSGKIGDAHLLLGNPALLKQHQVNITEGETLINEQAQRGITPVLLAANGHIIAVFSIH
DPLRADSVSALQRLHQQGYQLVMLTGDNPLTAKSIAKEAGIDQVIAGVLPDGKAEAIKKL
QAQGKRVAMIGDGINDAPALAQADVGIAMGGGSDIAIETAAITLMRHSLHGVADALALSN
ATLRNMKQNLLGAFIYNSLGIPIAAGVLYPLTGTLLSPVVAGAAMALSSITVVSNANRLL
RFKPKNQ