Protein Info for HER17_RS15485 in Pectobacterium carotovorum WPP14

Annotation: spermidine N1-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 191 PF13302: Acetyltransf_3" amino acids 8 to 142 (135 residues), 81.8 bits, see alignment E=2.8e-26 PF13420: Acetyltransf_4" amino acids 13 to 157 (145 residues), 27.7 bits, see alignment E=9.3e-10 PF13523: Acetyltransf_8" amino acids 16 to 146 (131 residues), 28.9 bits, see alignment E=2.9e-10 PF00583: Acetyltransf_1" amino acids 34 to 141 (108 residues), 65.9 bits, see alignment E=1.4e-21 PF13673: Acetyltransf_10" amino acids 47 to 147 (101 residues), 28.2 bits, see alignment E=5.8e-10 PF13508: Acetyltransf_7" amino acids 59 to 143 (85 residues), 35.6 bits, see alignment E=3.5e-12 PF08445: FR47" amino acids 88 to 143 (56 residues), 22.7 bits, see alignment E=2.8e-08

Best Hits

Swiss-Prot: 83% identical to ATDA_ECOLI: Spermidine N(1)-acetyltransferase (speG) from Escherichia coli (strain K12)

KEGG orthology group: K00657, diamine N-acetyltransferase [EC: 2.3.1.57] (inferred from 97% identity to pwa:Pecwa_3190)

MetaCyc: 83% identical to spermidine N-acetyltransferase (Escherichia coli K-12 substr. MG1655)
Diamine N-acetyltransferase. [EC: 2.3.1.57]; 2.3.1.57 [EC: 2.3.1.57]; 2.3.1.57 [EC: 2.3.1.57]

Predicted SEED Role

"Spermidine N1-acetyltransferase (EC 2.3.1.57)" in subsystem Polyamine Metabolism (EC 2.3.1.57)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.57

Use Curated BLAST to search for 2.3.1.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (191 amino acids)

>HER17_RS15485 spermidine N1-acetyltransferase (Pectobacterium carotovorum WPP14)
MSGTSNSVRLRPLERDDLTFVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERR
FIIEHEQTKVGLVELVEINHIHRRAEFQIIIDPAYQGKGYASAAAKLAMDYGFSVLNLYK
LYLIVDKENKKAIHIYSKLGFEVEGELIHEFFINGEYRNTIRMCIFQHQYMAKHKTVTGT
GGEAPGSSLSQ