Protein Info for HER17_RS14280 in Pectobacterium carotovorum WPP14

Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1043 PF02837: Glyco_hydro_2_N" amino acids 60 to 227 (168 residues), 223.4 bits, see alignment E=4.3e-70 PF00703: Glyco_hydro_2" amino acids 229 to 344 (116 residues), 52.8 bits, see alignment E=1.6e-17 PF02836: Glyco_hydro_2_C" amino acids 346 to 640 (295 residues), 403.4 bits, see alignment E=1.4e-124 PF16353: LacZ_4" amino acids 650 to 738 (89 residues), 77.8 bits, see alignment E=1.6e-25 PF02929: Bgal_small_N" amino acids 770 to 1036 (267 residues), 191.9 bits, see alignment E=4.2e-60

Best Hits

Swiss-Prot: 62% identical to BGAL2_ENTCL: Beta-galactosidase 2 (lacZ) from Enterobacter cloacae

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 91% identity to eca:ECA1490)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1043 amino acids)

>HER17_RS14280 beta-galactosidase (Pectobacterium carotovorum WPP14)
MSDTVLSYPAHHHATLQEILARRDWENPACTHYQRLPAHPPFNSWRSMAAAQQDEPSQRL
RRLNGEWTFSYFTRPEAVPESWLQQDLPDSDTIPVPANWQLQGYDTPIYTNVKYPIPVNP
PYVPEDNPTGCYSLTFKVNHDWISRGQTRIIFDGVNSAFYLWCNGHWVGYSQDSRLPAEF
DIGRYLTTGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVTLLHKPAVHLSDIQLTTPL
SADFRHGTLDIQVKATFSEAEAKNHRIHAQLWRGNKLIGDTRQAFGSDIVDERGAYHDKS
FLRIDVPQPDLWSAEQPHLYRAVIALETAEGKLVEAEAYDVGFRKVEIRSGLLLLNGQPL
LIRGVNRHEHHPQHGQVMDEDTMRHDIMLMKQHNFNAVRCSHYPNHPLWYRLCDRYGLYV
VDEANIETHGMQPMNRLSDDPVWLPAYSERVSRMVQRDRNHPCIIIWSLGNESGYGANHD
ALYQWIKRHDPTRPVHYEGGGANSRATDIVCPMYARVDEDQPFPSVPKWSITKWVSMPDE
HRPLILCEYAHAMGNSLGGFARYWQAFRQYPRLQGGFIWDWVDQALNRRDEQGNAYWAYG
GDFGDMPNDRQFCLDGLVFPDRTPHPSLYEAQRAQQHIQFFWQAESPCELRVTSEYLFRH
TDNEQLNWSITLNGKMLAEGSLPLTLAPQATQTLTLLEALPTVDRAGELWLNVEVVQPKA
TAWSEANHRCAWDQWQLPTPLHLPEALCSGQRKPPVLHSSDAYFDIIQGEQRWRFNRQSG
WLEQWWTADTPTLLTPLQDQFVRAPLDNDIGISEVDRIDPRAWAERWKSAGLYQLQTQCV
AIQADQLADAVHIATEHVFSYAGQILLRSKKRWQIDAHGVMTVDIDVDAATILPSLARVG
LSCQLADIAPQVSWVGLGPHENYPDRQLAAQHGHWNLPLDDLHTPYIFPTENGLRCNTRT
LAYGKWIITGNFHFGLSRYGLAQLMTCTHHHLLKKEKGVWLNLDGFHMGIGGDDSWSPSV
HRDDLLTATHYHYRVALQHHQPY