Protein Info for HER17_RS13545 in Pectobacterium carotovorum WPP14

Annotation: adenylosuccinate lyase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 17 to 296 (280 residues), 167.4 bits, E=5.6e-53
• PF10397: ADSL_C
  • amino acids 365 to 442 (78 residues), 69.1 bits, E=3.5e-23

Best Hits

Swiss-Prot: 47% identical to YBN5_SCHPO: Uncharacterized protein C8E4.05c (SPBC8E4.05c) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 98% identity to pct:PC1_1483)

MetaCyc: 46% identical to trans-aconitate decarboxylase (Ustilago maydis)
RXN-19902 [EC: 4.1.1.113]

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• itaconate biosynthesis II (1/3 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (1/4 steps found)
• aromatic compounds degradation via β-ketoadipate (2/9 steps found)
• toluene degradation III (aerobic) (via p-cresol) (1/11 steps found)
• superpathway of aerobic toluene degradation (3/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (4/35 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.113 or 5.5.1.2

Protein Sequence (449 amino acids)

>HER17_RS13545 adenylosuccinate lyase family protein (Pectobacterium carotovorum WPP14)
MASNVLDSVLFRDSFGTPEMRAIFDDRELIRKYVEVEIALANAQARCGVIPEGAAAEITA
SCNADTLDFELLRHETEIVGYPILPLVHQISKQAGQSGGYVHWGATTQDIMDTAVVLQIR
DAFALIEADIDALRSILAGLAARYRNTPMAGRTHLQQALPITFGYKAAIWLDMFDRHAER
LAQMRPRVLVGEFAGAAGTLASLGDKGLAVQKALMAELGLGIPTSTWHVARDGFAEAVNL
LAVITGSLGKIGYDVMLMASNEFGELYEPFVKGRGASSTMPQKRNPISSELMLACSKGVR
QQAGLMLDAMVQDLERATGPWHAEWIAIPESFILSAGALHQAKFMLNGLIVDEKMMLKNL
NMTNGLIVAEAVMMGLAPYIGRQEAHDIVYDACRIVNEKGGTLADVLNAMPGVSSRLDPK
LIEDLTNPVNYLGSAPEMVDQVLNKTERK